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Method for extracting total triterpene of centella asiatica by enzymic process

A technology for extracting total glucosides of Centella asiatica and enzymatic method is applied in the field of medicine, which can solve problems such as increasing equipment investment, and achieve the effects of changing energy consumption, high extraction rate and improving extraction rate.

Active Publication Date: 2011-06-15
桂林普兰德生物科技有限公司
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  • Application Information

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Problems solved by technology

Ultrasonic extraction has higher extraction efficiency and requires more investment in equipment

Method used

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  • Method for extracting total triterpene of centella asiatica by enzymic process

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Effect test

Embodiment 1

[0023] Weigh 50kg of Centella asiatica hay, heat 250L of pure water in the extractor to 100°C, put the centella asiatica hay into the extraction tank after heating to inactivate the endogenous enzymes of Centella asiatica, during the inactivation process, the extraction tank The internal temperature is controlled at 95-100°C, after inactivation for 30 minutes, continue to heat at 100°C for 60 minutes, and collect the extract; then add 750L pure water to the extraction tank, control the water temperature at 40-50°C, adjust the pH to 6.0, add Cellulase, make the enzyme activity in the water reach 20-40u / ml, enzymatically hydrolyze for 1.5 hours, discharge the extract, collect the extract; combine the extract, separate the extract through a membrane with a molecular weight of 1500dalton, and intercept it into the solution Soluble protein and macromolecular organic matter are collected from the filtrate; the filtrate is then separated by a membrane with a molecular weight of 800dal...

Embodiment 2

[0026] Weigh 500kg of Centella asiatica hay, heat 2500L of pure water in the extractor to 100°C, put the centella asiatica hay into the extraction tank after heating to inactivate the endogenous enzymes of Centella asiatica, during the inactivation process, the extraction tank The internal temperature is controlled at 95-100°C, inactivated for 30 minutes, then kept at 100°C for 60 minutes, and the extract is collected; add 7500L of pure water to the extraction tank, control the water temperature at 40-50°C, adjust the pH to 6.0, and add fiber Suzyme, the enzyme activity in the water liquid reaches 20-40u / ml, enzymatic hydrolysis for 1.5 hours, the extract is discharged, and the extract is released; the extract is combined, filtered, the filtrate is first separated by a membrane with a molecular weight of 1500dalton, and retained in the solution Soluble protein and macromolecular organic matter are collected from the filtrate; the filtrate is then separated by a membrane with a ...

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Abstract

The invention provides a method for extracting total triterpene of centella asiatica by an enzymic process, which comprises: deactivating endogenous enzymes of centella asiatica; and performing enzymic extraction. The extraction steep is high, the process conditions are mild, the extraction rate is high, the original properties of active ingredients are retained, and the low energy consumption is lowered. By using membrane separation, purification and concentration, organic solvent residue can be avoided effectively, less waste water is produced in the process, the purity of the separated total triterpene of centella asiatica is high, and the extraction rate is high.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for enzymatically extracting asiaticosides. Background technique [0002] Centella asiatica (L.) Urban is the dry or rooted whole plant of the dicotyledonous plant Umbelliferae. Widely distributed in the south of the Yangtze River Basin, it is a commonly used Chinese herbal medicine in folk areas in Guangdong. In my country, Centella asiatica has been used externally and taken internally to treat diseases for more than 2,000 years. Centella asiatica is cold in nature, bitter in taste, and pungent, and has the effects of clearing heat and promoting dampness, promoting blood circulation to stop bleeding, detoxifying and reducing swelling. The whole plant mainly contains triterpenoids, polyacetylenes, volatile oils and other ingredients. The main components of triterpenoids include Asiaticoside (Molecular formula C 48 h 78 o 19 , molecular weight 959.15CAS No: 16830-1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61P43/00A61P25/24A61P35/00A61P39/06A61P25/28A61P17/02
Inventor 吕光政叶勇蒙汉富
Owner 桂林普兰德生物科技有限公司
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