Application of bdellovibrio mixture in preparing turbot red bill disease preventing and curing bactericide

A technology of Bdellovibrio and its mixture, which is applied in the field of application of the mixture of Bdellovibrio and Swimming Body in the prevention and treatment of turbot redmouth disease, can solve food safety and other problems, and achieve effective application and no pathogenic bacteria Resistant, highly effective effects

Inactive Publication Date: 2011-04-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies of various food safety problems caused by the abuse of antibiotics in fish farming, the present invention provides an application of a mixture of Bdellovibrio in the preparation of a bacterial agent for preventing and treating turbot redmouth disease

Method used

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  • Application of bdellovibrio mixture in preparing turbot red bill disease preventing and curing bactericide
  • Application of bdellovibrio mixture in preparing turbot red bill disease preventing and curing bactericide

Examples

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Embodiment 1

[0026] The preparation of embodiment 1 bacterial agent

[0027] The Bdellovibrio strain used was Bdellovibrio BDM01. The Bdellovibrio sp. BDM01 was preserved in the China Center for Type Culture Collection on April 28, 2008, and the preservation number is CCTCC NO: M 208066; after it was negatively stained, its morphology was observed under an electron microscope: Bdellovibrio sp. The bacteria BDM01 is single-celled, arc-shaped, with a size of 1.7×1.0 μm, terminal flagella, and the length of the flagella is 3.5 μm; it can form a transparent circular phagocytosis with a diameter of 3-4 mm when cultured at 28°C for three days by double-layer plate method. plaque.

[0028] Preparation of Bdellovibrio Bdeloidoplasts: Inoculate 1mL conical flask with 100mL normal nutrient broth (NB) medium (peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, pH7.4) for 10 9 cfu / mL Aeromonas hydrophila (Aeromonashydrophila, purchased from Guangdong Provincial Institute of Mi...

Embodiment 2

[0032] Select juvenile turbot with healthy physique and uniform individual size, with an average weight of 55±2.5g, according to 300 fish / m 2 The breeding density was put into the pond, and the experimental period of each breeding pond was 4 months.

Embodiment 21

[0034] Normal running water (using external drainage, the amount of water exchanged every day and night is 8 running water), and the conventional turbot pellet feed (factory-produced breeding method) is put in, and no bacterial agent is added.

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Abstract

The invention discloses application of a bdellovibrio mixture in preparing a turbot red bill disease preventing and curing bactericide. The bdellovibrio is the bdellovibrio sp. BDM01. The turbot red bill disease preventing and curing bactericide is applied as follows: splashing the turbot red bill disease preventing and curing bactericide into the water body to ensure that the final concentration of the bdellovibrio mixture in the water body is 10-107 pfu / ml, or soaking the turbot red bill disease preventing and curing bactericide for feeds for 15-45 min to ensures that the concentration of the bdellovibrio mixture in the turbot red bill disease preventing and curing bactericide f is 10-107 pfu / ml. After being applied to preparing the turbot red bill disease preventing and controlling bactericide, the bdellovibrio mixture can effectively prevent and cure turbot red bill diseases, and has the advantages of fine safety, environmental protection, no pathogenic bacteria resistance generation and good application prospect in field of aquatic product animal cultivation.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the application of a fungus, in particular to the application of the mixture of bdellovibrio bdelloplast and swimming body in the prevention and treatment of turbot redmouth disease. Background technique [0002] Turbot (Scophthalmus maximus) is a rare and valuable flounder native to Europe. It has the advantages of adapting to life in low water temperature, fast growth, good meat quality, great breeding and market potential, etc., and has successively become one of the excellent marine cultured fish developed by European countries. Introduced to China in 1992, it has become an important cultured species along the northern coast of China. Turbot grows rapidly, and it only takes 10-12 months from spawning and hatching to growing commercial fish (0.8-1 catties) for sale. The investment in the year will benefit the profit in the year, and it has high economic value, high feed con...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00A61K35/74A61P31/04
Inventor 蔡俊鹏鲍星
Owner SOUTH CHINA UNIV OF TECH
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