Preparation method of anthocyanin monomer cornflower-3-glucoside

Abstract

The invention relates to a preparation method of anthocyanin monomer cornflower-3-glucoside. The preparation method comprises the following steps of: taking blackberries as a raw material, pulping, leaching and concentrating, removing impurities and purifying to obtain the anthocyanin monomer cornflower-3-glucoside. In the preparation method, the cornflower-3-glucoside is purified by utilizing a high speed counter current chromatograph; a solvent system of the cornflower-3-glucoside consists of n-butyl alcohol, organic acid and water; and the material cost is low and the toxicity is less, therefore, the cost is reduced, the recycling utilization rate of an upper phase and the operating flexibility and continuity of a device are enhanced, the residues of toxic and harmful ingredients in a product are reduced; the safety is high, the quality is controllable and the operation is simple and convenient.

Description

technical field [0001] The invention relates to the field of additives, in particular to a preparation method of anthocyanin monomer cyanidin-3-glucoside. Background technique [0002] Blackberry is one of the important species of the four large and small fruit trees widely cultivated in Europe and America. In 1993, the World Food and Agriculture Organization (FAO) recommended it as the third generation fruit in the world. Blackberry fruit is soft and juicy, sweet and sour, bright in color, mellow in flavor, and rich in nutrients, such as: anthocyanins, vitamin C, vitamin E, trace elements, amino acids, etc. Regular consumption has the magic of prolonging life, lightening body and retaining beauty Efficacy, known as "the fruit of life" in foreign countries, in recent years, the planting range of blackberries in my country has been expanding year by year, and the domestic and foreign markets are in short supply. [0003] According to the measurement, the content of anthocyani...

AI Technical Summary

Problems solved by technology

When solid-liquid column chromatography is used to separate anthocyanin monomer components in blackberries, silica gel is often used as a stationary phase to fill the chromatographic column, and the sample will not be completely absorbed due to adsorption on the stationary phase during the elution process of the mobile phase. eluted, resulting in the defect that the chromatographic column has dead adsorption on the product; at the same time, the chromatographic column has high requirements for the mobile phase solvent, which requires a chromatographically pure grade, and the cost is high

On the other hand, the solvent system used in the existing liquid-liquid countercurrent chromatographic separation technology at the laboratory research level is composed of five solvents, methyl tert-butyl ether, acetonitrile, n-butyric acid, trifluoroacetic acid and water, wherein The price of acetonitrile and trifluoroacetic acid is high, the toxicity is high, and the solvent is difficult to recycle and reuse, which is not conducive to the large-scale preparation of anthocyanins. Therefore, it remains to develop a kind of anthocyanin monomer cyanidin-3-glucose that can overcome the defects of the prior art The preparation method of glycoside