Microbial fermentation bed strain and manufacturing method thereof
A technology of microbial fermentation and strains, applied in the field of microbial fermentation, can solve the problems of high price, decline of strain vitality, and slow promotion of fermentation beds, etc., and achieve the effect of low cost, loose requirements, and good continuous stability
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Embodiment 1
[0034] A microorganism fermentation bed strain, its components and weight percentages are: 50% of Bacillus subtilis strain, 50% of Saccharomyces cerevisiae strain.
Embodiment 2
[0036] A microbial fermentation bed strain, the components and their weight percentages are: 70% of bacillus subtilis strains and 30% of saccharomyces cerevisiae strains.
Embodiment 3
[0038] A microbial fermentation bed strain, the components and their weight percentages are: 60% of subtilis strains and 40% of Saccharomyces cerevisiae strains.
[0039] A preparation method for microbial fermentation bed strains, comprising the following steps:
[0040] 1) Preparation of mother species
[0041] 1.1 Preparation of the Bacillus subtilis parent species: the parent species medium components and their weight percentages consist of: 1% glucose, 0.3% beef extract, 1% peptone, 0.5% salt, and finally add boiling water to adjust to the required volume, and the culture solution is Sterilize at 120°C for 20 minutes, cool to 35°C, inoculate the Bacillus subtilis strain at a weight ratio of 1 to 3%, and cultivate at a constant temperature of 30-35°C for 36-48 hours;
[0042] 1.2 Preparation of the mother species of Saccharomyces cerevisiae: the components of the mother species culture medium and their weight ratios are: saccharified wort: corn steep liquor = 100: 1, the ...
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