Method for north-south hybridization and reproduction regulation breeding of Lateolabrax

Abstract

The invention discloses a method for north-south hybridization and reproductive regulation breeding of Lateolabrax, which relates to the biotechnology breeding of Lateolabrax. The method comprises the following steps of: (1) taking a standby parent fish bred from the northern Lateolabrax seed as the male parent and a standby parent fish bred from the local wild Lateolabrax seed as the female parent; (2) breeding the male and the female parents to about 1500g to be used as the parent fishes; (3) artificially inducing the Lateolabrax through a reproductive regulation: controlling the time and the number of hatching of the Lateolabrax parent fishes by means of temperature, photoperiod and hormone injection, controlling the illumination time to 8h, reducing the temperature by 0.3 DEG C every day via a water cooler, and injecting CRH-A3 to the dorsal muscles of the parent fishes after anesthesia with allylguaiacol when the water temperature is reduced to 22 DEG C to induce the parent fishes to spawn, wherein the reproductive regulation induction period of the Lateolabrax is 7 days, and the Lateolabrax can be induced for several periods; (4) breeding the germ cells of the Lateolabrax; and (5) breeding juvenile fishes and breeding the juvenile fishes on a lower row when the sizes of the juvenile fishes reach more than 5cm. The invention has the advantage of breeding the new Lateolabrax seed which has the advantages of high temperature resistance, high growth speed, strong disease resistance and good quality by hybridizing northern and southern Lateolabraxes.

Description

Technical field: [0001] The invention relates to the biological breeding technology of perch, in particular to a method for breeding new strains of perch. Background technique: [0002] The current traditional breeding methods for sea bass are as follows: [0003] 1. Source selection of broodstock: finished perch fish cultured in local sea cages; [0004] 2. Breeding of broodstock and selection before artificial induced production: select the perch with strong physique, good shape and color, typical biological characteristics and weight over 1500g as the broodstock; [0005] 3. Artificial induced labor: broodstock were anesthetized with eugenol and injected with CRH-A into the dorsal muscle 3 Induce the broodstock to lay eggs, and after induced spawning, the broodstock are stored in the nursery pond according to (♀:♂=3:1), stimulated by running water, the broodstock spawns and ovulates, and collects the fertilized eggs; [0006] 4. Incubation of fertilized eggs of perch: ...

AI Technical Summary

Problems solved by technology

[0009] The disadvantage is that this non-selective breeding method cannot overcome the defects of the inbreeding of sea bass through generations, which makes the fis