Diagnosis model and diagnosis kit for peripheral blood gene of liver cancer

Abstract

The invention relates to a diagnosis model and a diagnosis kit for the peripheral blood gene of liver cancer. The diagnosis model comprises the expression level range indexes of 9 genes which are CXCR4, SPAG9, FOS, ANXA 1, CALR, IL8, PFN1, GPC3 and HGF; the kit comprises reagents which can detect the difference of expression levels of the 9 genes, namely the CXCR4, SPAG9, FOS, ANXA 1, CALR, IL8, PFN1, GPC3 and HGF from those of healthy people; and preferably, the reagents comprise an RNA extraction reagent and a multi-RT-PCR reagent, wherein the multi-RT-PCR reagent comprises SEQ ID No.1-18. The diagnosis accuracy of the diagnosis model established by the invention on five groups of peripheral blood samples is 76.0 percent.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, in particular to a liver cancer peripheral blood gene diagnosis model and a diagnosis kit. Background technique [0002] Primary hepatocellular carcinoma (hepatocellular carcinoma), referred to as liver cancer, is one of the common malignant tumors in my country, and its mortality rate ranks third among malignant tumors of the digestive system. The onset of liver cancer is hidden, and its typical symptoms and signs generally appear in the middle and late stages. Clinically, surgery, radiotherapy, chemotherapy, etc. are usually used. Discovery, early diagnosis, early treatment. At present, the early screening of liver cancer relies on serum alpha-fetoprotein detection and B-ultrasound examination to monitor high-risk groups. The combined inspection of these two methods enables some liver cancers to be diagnosed at the subclinical stage, thereby significantly improving the five-year surv...

AI Technical Summary

Problems solved by technology

At present, there have been many studies on related differential genes in tissues of patients with liver cancer, but the expression of related genes in tissues is mostly inconsistent with that in peripheral blood, and the acquisition of liver cancer tissues is invasive.

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