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Method for refrigerated storage of heterorhabditis bacteriophora entomopathogenic nematodes in cold areas

A technology of entomopathogenic nematodes and storage methods, which is applied in the field of variable temperature and frozen storage of bacteriophage Heterobacillus entomopathogenic nematodes in cold regions, can solve the problems of short storage period, difficult long-distance transportation, weak pathogenicity, etc., and achieve good results Effect

Inactive Publication Date: 2013-01-16
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of short storage period, difficult long-distance transportation, low survival rate and weak pathogenicity of entomopathogenic nematodes stored at low temperatures above 0°C, and to provide a method for use in cold regions. Method for frozen storage of entomopathogenic nematodes

Method used

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Examples

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Effect test

specific Embodiment approach 1

[0015] Specific embodiment 1: The specific operation steps of this embodiment for the frozen storage method of bacterophilic Heterobacteriaceae entomopathogenic nematodes in cold regions are as follows:

[0016] Step 1. Prepare the storage medium and storage bag: the storage medium is vermiculite, pass the vermiculite through a 40-60 mesh sieve, remove the fines, keep the ventilation, then sterilize with damp heat at 121°C for 25-30min, add water to the vermiculite water content 45-60%; the storage bag is 80-100 mesh nylon mesh bag, the size is 10×20cm, put the storage medium into the storage bag;

[0017] Step 2, inoculation: inoculate the entomopathogenic nematode (infection stage larvae IJs) of the newly propagated entomopathogenic nematodes (infection stage larvae IJs) stored at 10°C into the storage medium vermiculite; press 10 4-5 The dose of IJs / g vermiculite inoculates the larvae of the infection stage;

[0018] Step 3. Variable temperature frozen storage: Put the ino...

specific Embodiment approach 2

[0021] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the vermiculite in step 1 passes through a 50-mesh sieve. Other steps and parameters are the same as those in Embodiment 1.

[0022] of this embodiment

specific Embodiment approach 3

[0023] Embodiment 3: This embodiment is different from Embodiment 1 in that the nylon mesh bag in step 1 is 80 mesh. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention provides a method for the refrigerated storage of heterorhabditis bacteriophora entomopathogenic nematodes in cold areas, which relates to the technical field of biological refrigerated storage and solves the problems of short storage period, difficult long-distance transportation, low survival rate and low pathogenicity of the conventional low temperature (over 0 DEG C) storage of entomopathogenic nematodes. The method comprises the following steps of: 1) preparing storage media and storage bags; 2) inoculating; 3) performing refrigerated storage at different temperatures; 4) defreezing; and 5) isolating nematodes. The method for the refrigerated storage of the heterorhabditis bacteriophora entomopathogenic nematodes in the cold areas performs the refrigerated storage at the different temperatures on the heterorhabditis bacteriophora entomopathogenic nematodes which are peculiar to high-cold areas, breaks the limitations of current production scale and application areas of the entomopathogenic nematodes to ensure a storage period of 5 to 6 months, a rate of the recovering of the nematodes from the storage medium of 90 percent, a defrozen nematode survival rate of over 80 percent and a soil insect prevention and control effect of over 80 percent, solves the problems of unfavorable long-distance transportation and obviously decreased pathogenicity of the nematodes which are stored at the temperature of over 0 DEG C for long time and achieves a good effect of biologically preventing and controlling destructive insects in agricultural productive practice.

Description

technical field [0001] The invention relates to the technical field of biological freezing storage, in particular to a variable temperature freezing storage method for Heterorhabditis bacteriophora entomopathogenic nematodes in cold regions. Background technique [0002] As we all know, entomopathogenic nematodes (Entomopathogenic nematodes) is a kind of potential biological control factor developed in the early 20th century, which refers to a class of parasitic nematodes that carry pathogenic symbiotic bacteria in their bodies and can cause insect pathogenicity. It has been successfully used to control more than 250 kinds of pests such as grubs, mushroom flies, small wood beetles, litchi wood beetles, bald beetles and citrus weevils. Precisely because entomopathogenic nematodes are broad-spectrum, highly efficient, and safe for humans, animals, and the environment, more than 40 countries, including the United States, Australia, Germany, New Zealand, Canada, Japan, Russia, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 李春杰许艳丽
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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