Rice secretary type thioredoxin gene and application thereof

A thioredoxin, secreted technology, applied in the field of genetic engineering, can solve problems such as plant aging, crop yield reduction, oxidative damage, etc., and achieve the effects of reducing hydrogen peroxide content, delaying rice aging, and increasing crop yield

Inactive Publication Date: 2010-09-22
HEBEI NORMAL UNIV
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  • Abstract
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AI Technical Summary

Benefits of technology

This patented technology allows for increased production from crops when overexpressed or reduced during certain stages of their life cycle (such as ripening).

Problems solved by technology

This patents discusses how certain chemicals called thioremedoxins play key roles during various stages of growth and maintenance within plants like roots, shoots, stalk tips, seed pods, fruits, vegetables, flowers, and even nuts. These compounds may be released into surrounding environments when exposed to harsh environmental factors causing degradations over time. Overall, these findings help us better understanding why plants grow old faster than before by studying them at least partially due to its ability to reduce free radicals produced from other metabolites present naturally throughout our bodies.

Method used

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  • Rice secretary type thioredoxin gene and application thereof
  • Rice secretary type thioredoxin gene and application thereof
  • Rice secretary type thioredoxin gene and application thereof

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Experimental program
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Embodiment Construction

[0019] 1. Acquisition of genes

[0020] This thioredoxin is a protein that the applicant found during two-dimensional electrophoresis, and the expression of rice root apoplasts is up-regulated under salt stress conditions. The Accession No. of the protein is gi|3915131, and its gene sequence number is AK121423. In order to obtain the gene, the applicant extracted the RNA of wild-type rice (Oryza sativa), and used primers 5'-CCATGGCCGCCGAGGAGGGA-3' and 5'-CCATGGCGGCAGAAGCAGATGCAGCAGTG-3' for RT-PCR amplification. The reaction system was as follows: ultrapure water 31ul, 10×Pyrobest Buffer II 5ul, dNTP Mixture 4ul, upstream and downstream primers 4ul, Pyrobest DNA Polymerase 0.2ul, cDNA 2ul. The PCR program is: 5 minutes at 94°C, 30 seconds at 94°C, 30 seconds at 61°C, 50 seconds at 72°C, 27 cycles from the second to fourth steps, and 10 minutes at 72°C. The PCR product was subjected to electrophoresis, recovered from the gel, connected to the T carrier, and the full-length nuc...

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Abstract

The invention discloses a rice secretary type thioredoxin gene, coded proteins thereof and application thereof and aims to provide the rice secretary type thioredoxin gene, the coded proteins thereof and a method for improving antioxidant activities of plants and delaying plant senescence by using the gene. The nucleotide sequence of the rice secretary type thioredoxin gene provided by the invention is a No.1 DNA sequence in a sequence table; and the amino acid sequence coded by the secretary type thioredoxin gene is a No.2 amino acid residue sequence in the sequence table. Experiments show that when the gene is excessively expressed in the rice, hydrogen peroxide content of a plant can be reduced and chlorophyll content can be improved in a normal state or in the presence of salts, so a rice senescence process is delayed. The rice secretary type thioredoxin gene, the coded proteins thereof and the application thereof have important application in aspects of improving the anti-oxidation and anti-aging performance of rice by using molecular breeding.

Description

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Claims

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Application Information

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Owner HEBEI NORMAL UNIV
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