Method for preserving zostera marina seeds and zostera caespitosa seeds
A preservation method and a technology for the algae, applied in the seed and rhizome treatment, application, agriculture and other directions, can solve the problem of long-term preservation of algae and clump algae seeds, increased seed cumulative germination rate, influence on spring sowing, etc. problems, to restore the marine ecological environment, prolong the preservation time, high vitality and quality
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Embodiment 1
[0034] According to the above formula, 47.12 grams of artificial seawater is added to 1000 ml of pure water to prepare high-salinity artificial seawater with a salinity of 45 psu (that is, the weight ratio of artificial seawater to artificial seawater is 45‰). Every part of 300 eel algae seeds, get two parts and put them into two Erlenmeyer flasks containing artificial seawater with a salinity of 45psu respectively, place them at a temperature of 4°C and 14°C respectively, and the photoperiod is D:L=12: 12. The light intensity is 20μmol m -2 the s -1 The purpose of inflating is to keep the seeds floating in the water without sinking to the bottom of the water. L:D=12:12 is the ratio of light time to dark time, that is, 12 hours of light and 12 hours of darkness, simulating day and night switching. Observe the status of the preserved seeds every week, and replace the artificial seawater with equal salinity every 5 to 7 days, so as to keep the seeds in a synchronized dormant st...
Embodiment 2
[0039] Similarly, 75.27 grams of artificial seawater is added to 1000 ml of pure water to prepare high-salinity artificial seawater with a salinity of 70 psu (that is, the weight ratio of artificial seawater to artificial seawater is 70‰). Every part of 300 eel algae seeds, get two parts and put it into two Erlenmeyer flasks that are filled with artificial seawater with a salinity of 70psu respectively, place them at a temperature of 4°C and 14°C respectively, and the photoperiod is D:L=12: 12. The light intensity is 20μmol m -2 the s -1 Store in aerated environment under environmental conditions, replace with artificial seawater of equal salinity every 5 to 7 days, check the status of the seeds every month and count the germinated seeds for a total of 7 months. Table 4 shows the records of the cumulative germination rate of the seeds of Z. chinensis preserved at a salinity of 70 psu and a temperature of 4°C and 14°C.
[0040] Table 4
[0041] time
Cumulative g...
Embodiment 3
[0050] According to the above formula, 47.12 grams of artificial seawater is added to 1000 ml of pure water to prepare high-salinity artificial seawater with a salinity of 45 psu (that is, the weight ratio of artificial seawater to artificial seawater is 45‰). For each 300 grains of Z. clumpingensis seeds, two parts are put into two Erlenmeyer flasks filled with artificial seawater with a salinity of 45psu respectively, placed at a temperature of 4°C and 14°C respectively, and the photoperiod is D:L=12 : 12, the light intensity is 20μmol m -2 the s -1 The purpose of inflating is to keep the seeds floating in the water without sinking to the bottom of the water. L:D=12:12 is the ratio of light time to dark time, that is, 12 hours of light and 12 hours of darkness, simulating day and night switching. Observe the status of the preserved seeds every week, and replace the artificial seawater with equal salinity every 5 to 7 days, so as to keep the seeds in a synchronized dormant s...
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