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FITC-doped silica fluorescent nanoparticle and preparation method thereof

A fluorescein isothiocyanate, fluorescent nanotechnology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as dye leakage, prevent leakage, improve chemical stability and photostability. Effect

Inactive Publication Date: 2010-08-18
SHANGHAI UNIV
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Problems solved by technology

[0005] At present, the existing technology is to directly dope FITC with fluorescein isothiocyanate to synthesize silica core-shell nanoparticles, but this method is easy to cause dye leakage

Method used

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  • FITC-doped silica fluorescent nanoparticle and preparation method thereof
  • FITC-doped silica fluorescent nanoparticle and preparation method thereof
  • FITC-doped silica fluorescent nanoparticle and preparation method thereof

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Embodiment Construction

[0029] 1. Instruments and reagents: FITC, APTMS, surfactant TritonX-100, etc. are all analytically pure and purchased from Sigma; mouse neural stem cells are provided by the School of Life Sciences, Shanghai University; other reagents are from Shanghai Sinopharm Chemical Reagent Co., Ltd. purchased, all analytically pure. The water used was ultrapure water.

[0030] FV1000 laser confocal scanning microscope (Olympus, Japan); SPM-9600 atomic force microscope (Shimadzu, Japan); JSM-2010F transmission electron microscope (JEOL, Japan); F-7000 fluorescence spectrophotometer (Hitachi, Japan); U-3010 ultraviolet-visible spectrophotometer (Japan Hitachi Company); CR21GII high-speed centrifuge (Japan Hitachi Company); PHS-3C precision pH meter (Shanghai Lei Magnetic Instrument Factory).

[0031] 2. FITC-APTMS / SiO2 2 Preparation and Characterization of Core-shell Fluorescent Nanoparticles

[0032] Please refer to the reference (SantraS, 2006) for the method of reacting FITC and APTM...

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Abstract

The invention relates to a silica fluorescent nanoparticle and a preparation method thereof, in particular to an FITC-doped silica fluorescent nanoparticle and the preparation method thereof. The method particularly comprises the preparation of a precursor solution and other steps. The FITC is reacted with 3-aminopropyl trimethoxysilane to prepare a precursor, and then the novel FITC-doped silica fluorescent nanoparticle is prepared by a water-in-oil microemulsion method. The prepared nanoparticle is uniformly spherical, has good monodispersity and light stability, difficultly generates dye leakage, has good response to the pH value and can be applied to the real-time monitoring of the pH value of cells.

Description

technical field [0001] The invention relates to a silicon dioxide nano fluorescent nano particle and a preparation method thereof, in particular to a silicon dioxide fluorescent nano particle doped with fluorescein isothiocyanate FITC and a preparation method thereof. Background technique [0002] Direct in situ monitoring of living cells can truly understand the life activities of living cells, but it is difficult to achieve this with traditional methods. Fluorescent nanoparticles can directly enter a single living cell, and nanobiological probes based on fluorescent nanoparticles can monitor active substances such as Ca in living cells with high sensitivity and high selectivity. 2+ , H + , RNA, etc. [0003] h + Ions play an important role in regulating the life activities of organisms, and the occurrence of some life phenomena is often accompanied by intracellular H + Therefore, the measurement of pH at the single-cell level is of great significance to the interpretat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
Inventor 谢春娟吴明红尹东光李剑张礼刘斌虎
Owner SHANGHAI UNIV
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