Local administration of chicken yolk immune globulins (igy) to treat and prevent fungal infections

A technology of fungi, Candida albicans, applied in the diagnosis of fungi, drugs for the prevention and/or treatment of all types of fungal infections, and the prevention or treatment of such diseases, which can solve the problems of loss of activity and unsuitability for long-term use in humans

Inactive Publication Date: 2010-07-28
ОГЕРУП БЕНГТ
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, they elicit anti-IgG antibodies against themselves, which causes a loss of their activity
Therefore, they are not suitable for long-term use in humans

Method used

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  • Local administration of chicken yolk immune globulins (igy) to treat and prevent fungal infections
  • Local administration of chicken yolk immune globulins (igy) to treat and prevent fungal infections
  • Local administration of chicken yolk immune globulins (igy) to treat and prevent fungal infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Preparation of fungi

[0092] Candida species isolated from infected patients were used in in vitro experiments to demonstrate the preventive potential of egg immunoglobulins isolated from chickens hyperimmunized with fungal antigens.

[0093] The fungi were cultured in 500 ml culture flasks containing 100 ml of 2% glucose supplemented with amino acids, 0.15% yeast nitrogen source, and 0.5% ammonium sulfate. The flasks were shaken at 200 rpm at 37°C for 24 hours in a rotary incubator. The fungi were also cultured on Candida plates used to detect Candida colonization in patient samples.

[0094] Preparation of anti-Candida IgY immunoglobulin

[0095] Formaldehyde-killed Candida albicans washed in saline, Candida glabrata , Candida krusei mother and Candida parapsilosis suspension and freeze. Separate groups of chickens were immunized with each Candida species. Use 10 per chicken per immunization 7 Candida. White Leghorn chickens were immunized intramuscular...

Embodiment 2

[0098] Cell Adhesion Assay

[0099] Adhesion to the mucosal epithelium is considered the first stage of infection. In this example, epithelial cells and Pseudomonas aeruginosa (as a representative of bacteria) were used to evaluate adhesion.

[0100] Fresh cells cultured for 24 hours were washed by centrifugation in PBS and resuspended in PBS with IgY from eggs immunized with Pseudomonas or IgY from non-immunized chickens (100:1 ratio). Alternatively mix with medium without IgY at all and incubate at 37°C for 2 hr. After incubation, the culture was filtered through a 45 gm filter to remove any non-adherent cells, washed and resuspended in PBS.

[0101] Adhesion was assessed by microscopy at 400X, using a graded micrometric grid to aid in accurate counting. The adhesion rate is expressed as the percentage of cells visibly adhered to Pseudomonas aeruginosa.

[0102] The results showed that the adhesion of Pseudomonas aeruginosa to epithelial cells was reduced by more than 50...

Embodiment 3

[0111] Example 3: Freeze-drying and concentration of IgY extracts

[0112] double freeze drying

[0113] 20 freeze-dried vials each containing 4 ml of the IgY extract of the present invention were provided. 8.5 mg of lactose was added to 10 of the bottles. 34 mg of lactose was added to the remaining 10 vials. Vials were freeze-dried according to standard protocols.

[0114] After drying, two vials of each lactose concentration were set aside and recorded as "single freeze-dried". The product in the remaining bottle was dissolved in distilled water according to the table below. For each lactose concentration ("high" or "low"):

[0115] Add 4ml of water to two of the bottles

[0116] Add 2ml of water to two of the bottles

[0117] Add 1ml of water to two of the bottles

[0118] Add 0.5ml of water to two of the bottles

[0119] The amount of water added was marked on the bottle (ie 4, 2, 1 and 0.5 respectively). Sixteen vials containing reconstituted IgY were lyophili...

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Abstract

The present invention relates to a composition comprising IgY antibodies against at least two different fungi, the use of the composition for the preparation of a pharmaceutical especially for prophylaxis and / or therapy of all kinds of fungal infections, such as conditions caused by organisms belonging to the Candida genus or Aspergillus genus, especially of fungi that are less sensitive to antimycotica and a diagnotstic method using the IgY antibodies. Further it relates to immunisation of birds with a combination of at least two different species of fungi to the Candida genus or Aspergillus genus and a diagnostic method for fungi. It also relates to relates to a novel method for treatment and / or prophylaxis of fungal infections, especially infections by fungi which have lowered sensitivity or resistance to antimycotics. The method is both safe and effective. This is achieved by using IgY directed against fungi, in particular against Candida species, which have a lowered sensitivity or resistance against antimycotics. The specific IgY has been obtained by hyper immunising birds with one or several fungi as antigen in order to stimulate the production of immune globulins (IgY) against such fungi. The present invention also relates to a pharmaceutical product from eggs of birds containing immune globulin or a fragment thereof, which can be combined with other preparations, nutritients or pharmaceuticals for simultaneous, separate or sequential use in the prophylaxis or therapy of gastrointestinal infections in newborn infants.

Description

technical field [0001] The present invention relates to the use of a composition comprising IgY antibodies against at least two different fungi for the preparation of a medicament, in particular for the prevention and / or treatment of all types of fungal infections, for example caused by the A disease caused by organisms of the genus Candida or Aspergillus (especially fungi that are less susceptible to antifungal agents). It also involves immunizing birds with at least two different fungal species. [0002] The present invention also relates to methods of preventing or treating fungal infections of all kinds (eg Candida species, Aspergillus species, etc.) and methods of diagnosis using IgY antibodies in any human being suffering from such diseases. Furthermore, it also relates to diagnostic methods for fungi. Background technique [0003] Fungal infections can be recurrent, severe, and life-threatening for some patients. For example, patients with APS1, patients treated wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02C07K16/14A61K39/395A61P31/10
CPCC07K16/02C07K16/14C07K2317/11A61K9/19A61K9/0056A61K2039/505C07K2317/23A61K9/0034A61P1/00A61P1/02A61P1/04A61P1/10A61P1/12A61P1/14A61P11/00A61P11/02A61P11/14A61P13/02A61P13/08A61P13/10A61P15/00A61P15/08A61P15/10A61P17/00A61P17/04A61P17/06A61P17/10A61P19/02A61P21/00A61P21/02A61P25/04A61P25/06A61P25/18A61P25/20A61P25/22A61P25/24A61P27/02A61P27/16A61P29/02A61P3/10A61P31/10A61P3/04A61P3/08A61P37/08A61P5/14A61P5/38A61P7/10
Inventor 安德斯·拉尔森汉斯·科尔贝里
Owner ОГЕРУП БЕНГТ
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