Styrene-degrading bacteria MJ001 and separating method thereof

A technology of MJ001 and separation method, applied in the field of styrene degrading bacteria MJ001 and its separation, can solve problems such as the ability to degrade styrene that has not yet been found, achieve strong degradation ability, improve purification ability, and solve the effect of environmental pollution problems

Inactive Publication Date: 2010-02-03
天津市天人合环保技术研发中心
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Benefits of technology

This patented method uses specific types of organic compounds called benzene or other substances found naturally on plants like bananas (fava beans) during production processes such as harvesting them from their roots. These chemical agents can break up harmful molecules into smaller parts while still providing nutrients needed by certain fungi. They are effective at reducing levels of volatile organics released through decomposition reactions involving these germs without causing any negative side effect. Overall this technology helps prevent damage caused by excessive amounts of environmentally hazardous materials used in industrial manufacturing processes.

Problems solved by technology

The technical problem addressed in this patented text relates to environmental concerns caused by strayalkenum (a type of plant called syringene) that occurs during the process of producing polymers like polystyrenes or vinyl chloride from benzene monomethanesulfonate solution. This compound causes damage through exposures to sunlight over time due to its high vapor pressure properties. To solve these issues, researcher explored ways to prevent it from entering into environments where they could affect humans' well-beings and potentially lead to negative publicity outcomes.

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  • Styrene-degrading bacteria MJ001 and separating method thereof
  • Styrene-degrading bacteria MJ001 and separating method thereof
  • Styrene-degrading bacteria MJ001 and separating method thereof

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Embodiment Construction

[0026] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0027] A styrene-degrading bacterium MJ001, Gram stain negative, facultative aerobic, optimum growth temperature 37 ℃, optimum pH 6.4-7.4, trisaccharide iron test negative, oxidase reaction positive, 16S rDNA sequence and green Pseudomonas P.aeruginosa has 100% homology, and the strain is preserved in: General Microbiology Center of China Committee for Culture Collection of Microbial Cultures, the preservation number is: 3042, and the preservation date is: April 28, 2009. The biological classification of the strain is named: Pseudomonas aeruginosa / Pseudomonas aeruginosa.

[0028] A method for isolating styrene-degrading bacteria MJ001, the steps are:

[0029] (1) Isolation of styrene-degrading bacteria MJ001:

[0030] ①Put a certain amo...

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Abstract

The invention relates to styrene-degrading bacteria MJ001 and a separating method thereof. The styrene-degrading bacteria MJ001 is characterized in that: gram faerbung is feminine; the bacteria is facultative and aerobic; the most proper growth temperature is 37 DEG C; the most proper pH value is 6.4-7.4; the triple sugar iron experiment is feminine; the oxydase reaction is male; and 16S rDNA sequence and Pseudomonas aeruginosa (P. aeruginosa ) have 100% homology. The separating method comprises the following steps of: (1) activating activated sludge; (2) inoculating bacteria liquid into liquid culture medium which takes styrene as only one carbon source; (3) coating culture solution on the liquid culture medium which takes styrene as only one carbon source, selecting larger single colonyto be microscopically examined, and storing; and (4) repeating the cultivation period of the step (2) and the step (3) at 2-4 times, and finally separating to obtain bacterial strain which cultures bytaking the styrene as only one carbon source. The invention obtains the characteristic of the styrene-degrading bacteria MJ001 by research, acknowledges the degrading mechanism of the strain, is goodfor improving the purifying capacity of microorganism, effectively solves the environment pollution problem of the styrene, and has significant meaning for purifying styrene-containing industrial waste water.

Description

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Claims

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Application Information

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Owner 天津市天人合环保技术研发中心
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