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Oligosaccharide probiotics

A technology of oligosaccharides and prebiotics, which is applied in the field of oligosaccharides and prebiotics, can solve the problems of difficult production techniques such as chemical structure separation and refinement, and achieve the effects of cheap raw materials, promoting growth, and promoting proliferation

Inactive Publication Date: 2010-02-03
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research and development work on oligosaccharides in the world is mainly neutral oligosaccharides, while there are relatively few studies on acid oligosaccharides.
In fact, neutral oligosaccharides have difficulties in production technology such as separation and purification due to their chemical structure and properties.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation of pectin oligosaccharides:

[0036] 1) Pass the hawthorn fruit or the residue after juicing through water (50-100° C.), extract for 3 hours, and use ethanol precipitation according to the conventional pectin recovery method to recover hawthorn pectin.

[0037] 2) Take an aqueous hawthorn pectin solution with a mass percentage concentration of 1%, adjust the pH to 3, and decompose at 110°C for 3 hours to obtain a hawthorn pectin decomposition product;

[0038] 3) Treat the obtained hawthorn pectin decomposition product with a hollow fiber membrane, recover the oligosaccharide mixture with a molecular weight below 6000 Daltons, recover the oligosaccharide mixture with a molecular weight of 200 to 6000 Daltons with a nanofiltration membrane, and use Bio - gel P-2 and Sephadex G-25 gel column separation to obtain pectin oligosaccharides with an overlap of 2-20.

[0039] (2) Preparation of oligosaccharide prebiotics

[0040] The oligosaccharide prebiotic i...

Embodiment 2

[0044] (1) Preparation of pectin acid oligosaccharides:

[0045] 1) Pass the hawthorn fruit or the residue after juicing through water (50-100° C.), extract for 3 hours, and use ethanol precipitation according to the conventional pectin recovery method to recover hawthorn pectin.

[0046]2) Take an aqueous hawthorn pectin solution with a mass percent concentration of 1%, adjust the pH to 3, and decompose at 110° C. for 3 hours to obtain a hawthorn pectin decomposition product;

[0047] 3) Treat the obtained hawthorn pectin decomposition product with a hollow fiber membrane, recover the oligosaccharide mixture with a molecular weight below 6000 Daltons, recover the oligosaccharide mixture with a molecular weight of 200 to 6000 Daltons with a nanofiltration membrane, and use Bio -gel P-2 and Sephadex G-25 gel column separation to obtain pectin oligosaccharides with an overlap of 2-20.

[0048] (2) Preparation of oligosaccharide prebiotics

[0049] The oligosaccharide prebiotic...

Embodiment 3

[0053] (1) Preparation of pectin acid oligosaccharides:

[0054] 1) After the apples are squeezed and processed, the juice residue is passed through an oxalic acid solution (pH 2-6, 50-90°C), extracted for 3 hours, filtered, and the filtrate is precipitated with ethanol according to a conventional pectin recovery method, and recovered to obtain apples pectin.

[0055] 2) Take an aqueous solution of apple pectin with a mass percent concentration of 2%, adjust the pH to 2, and decompose it at 95°C for 24 hours to obtain a decomposition product of apple pectin;

[0056] 3) The obtained apple pectin decomposition product is treated with a ceramic filter membrane, and the oligosaccharide mixture with a molecular weight of 6000 Daltons is recovered, and the oligosaccharide mixture with a molecular weight of 200 to 6000 Daltons is recovered with a nanofiltration membrane, and the Sephadex G Pectin oligosaccharides with a coincidence degree of 2-20 were separated by -25 gel column ch...

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Abstract

The invention relates to oligosaccharide probiotics. The invention adopts the following technical scheme: the oligosaccharide probiotics comprises 40-80 parts of pectic oligosaccarides, 40-80 parts offructo-oligosaccharides and 1-5 parts of protein by weight. Other accessories can be also added into the oligosaccharide probiotics, which are the mixtures of one or more of 5-20 parts of carbonhydrate, 0.2-2.0 parts of nitrogen nutrition components, 0.4-3.5 parts of fatty acid, 0.02-0.8 part of vitamins, 0.2-0.7 part of bioactive functional factors or 0.05-0.2 part of mineral elements. The oligosaccharide probiotics feature easy production method and easily obtained raw materials, have the antibacterial function and play a role of promoting the growth of bifidobacterium obviously.

Description

technical field [0001] The invention belongs to the field of food additives and food ingredients, and in particular relates to an oligosaccharide prebiotic containing pectin acid oligosaccharide with the effect of a prebiotic. Background technique [0002] With the development of gut microbiology research, the influence of gut microbiota on host health has been gradually elucidated, and the importance of the reproductive balance of gut microbiota to health has gradually been recognized. Probiotics in the intestinal tract such as bifidobacteria and lactic acid bacteria play an important role in purifying the intestinal tract, improving the body's nutrition and maintaining the health of the host. Prebiotics, as indigestible or indigestible food ingredients, can improve the balance of intestinal flora by selectively activating and increasing the growth and metabolism of beneficial intestinal microorganisms, thereby benefiting the health of human life. [0003] There are many t...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/308C12P19/14A23L33/125
Inventor 李拖平李苏红王娜郭梅
Owner LIAONING UNIVERSITY
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