Method for distributing mobile phase of chromatographic column and distributing device thereof

Abstract

A method for distributing the mobile phase of a chromatographic column and a distributing device thereof. The distributing method includes: adopting a circular distributor matching the cross-section of the prepared chromatographic column, and setting the fluid phase on the distributor along its center in a tree-shaped divergence outward. Distribution liquid path, the end of each fluid phase distribution liquid path is provided with a distribution hole, the length of the fluid phase distribution liquid path of the same specification from the center of the distributor to each distribution hole is equal; the fluid phase is from the center of the distributor Along the distribution path of each fluid phase, it is evenly distributed to each distribution hole, and then evenly distributed to the stationary phase of the chromatographic column. The invention can effectively eliminate the defect that the residence time of the mobile phase on the distribution system is seriously different with the increase of the diameter of the chromatographic column, and can realize the uniform initial distribution of the mobile phase entering the chromatographic column, and will not be affected by the flow of the mobile phase. High pressure delivery effects. The distribution device has a simple structural design, is not easy to be blocked, and is easy to clean and use repeatedly.

Description

technical field [0001] The invention belongs to the distributing technology of mobile phase in a chromatographic column for industrial preparation of chromatography, and in particular relates to a mobile phase distributing method and a distributing device especially suitable for a large-diameter chromatographic column. Background technique [0002] The main purpose of industrial preparative chromatography is to obtain pure substances from mixtures. In order to speed up the separation time and improve the separation efficiency, the amount of sample input to the preparative chromatographic device is large, resulting in a corresponding increase in the separation load of the preparative chromatographic column, and it is necessary to increase the column packing and increase the diameter and length of the preparative chromatographic column. , using a relatively large amount of mobile phase. After the liquid to be treated is injected into the chromatographic column, it moves in th...

AI Technical Summary

Problems solved by technology

But for the larger chromatographic column of diameter, especially the diameter of the chromatographic column of existing industrial preparation chromatogram increases gradually, the distribution uniformity of the fine distribution channel after entering the filter through a central inlet of the mobile phase on the distribution system will change with Decreases as the diameter of the column increases, eventually resulting in an uneven initial fluid distribution that affects the separation

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