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Method for culturing cephalotaxus excised embryos and regenerating plants

A technology for cultivating Chinese fir and embryos, applied in plant regeneration, botanical equipment and methods, gardening methods, etc., can solve the problems of short multiplication time, low emergence rate, and long seedling time, and achieve good growth and significant economic benefits Effect

Inactive Publication Date: 2009-12-30
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0003] The object of the present invention is to provide a kind of Tissue culture method of the isolated embryo culture and plant regeneration of Cedarwood; solve the long dormancy time of Cedarwood seeds, long seedling time and low emergence rate in the prior art, so it is difficult to obtain ideal The problem of germination rate and more robust seedlings, the buds cultivated by this method are healthy and strong, the proliferation time is short, the survival rate of mature seedlings is high after transplantation, and the seedlings grow robustly after survival and grow well, which has significant economic benefits

Method used

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  • Method for culturing cephalotaxus excised embryos and regenerating plants
  • Method for culturing cephalotaxus excised embryos and regenerating plants

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Embodiment

[0018] Material collection method: select the cloverleaf seeds with full grains of the year, wrap them with a damp cloth after picking, and store them temporarily at 4°C.

[0019] Medium:

[0020] The MS basic medium was prepared using the formula in "Tissue Culture and Application of Woody Plants" published by Beijing Higher Education Press and edited by Chen Zhenghua in 1986.

[0021] The preparation of the medium: the basic medium is MS, the growth hormone is IBA, NAA, IAA, ZE (zeatin), KT (phytohormone), and the Su (sucrose) is 25g / L in the medium, Ag + 7-7.5g / L, PH value 5.8, and activated carbon Ac as an appendage; the thickness of the culture medium is generally 1.4-1.6cm;

[0022] Bud induction medium→MS+ZE(2.0mg / L)+NAA(0.1mg / L)+IAA(1.0mg / L)+Ag + (7~7.5g / L)+Su(25g / L)+Ac(2~2.5g / L);

[0023] Bud Proliferation Medium→MS+ZE(2.0mg / L)+NAA(1.0mg / L)+IAA(1.0mg / L)+Ag + (7~7.5g / L)+Su(25g / L)+Ac(2~2.5g / L);

[0024] Rooting medium→1 / 2MS+NAA(0.5mg / L)+IBA(1.0mg / L)+KT(0.3mg / L)+Ag ...

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Abstract

The invention provides a method for culturing cephalotaxus excised embryos and regenerating plants, which belongs to the cephalotaxus culture technology. The method aims to solve the problem that the prior art is difficult to get ideal germination rate and more robust seedlings because of the long dormancy period of cephalotaxus seeds, long emergence time and low emergence rate. The method is characterized in that fresh plump seeds are selected as materials and subjected to induction culture; robust sprouts can be germinated from embryos after 15 to 20 days; seedlings can be formed and transplanted after 20 to 25 days of proliferation and 25 to 30 days of root induction; and the survival rate is as high as above 90 percent. The method has the advantage of high propagation factor, and surviving seedlings are good in growth, robust and upright.

Description

technical field [0001] The invention belongs to the cultivation technology of Cedarwood, and more specifically relates to a method for culturing isolated embryos of Cedarcus and plant regeneration. Background technique [0002] There are 9 species in total, 8 species in my country, including 3 species in Fujian Province. Its leaves resemble fir, but are soft and not prickly. The winter buds at the ends of the branches are arranged in a triangle. The tree is graceful, dignified and beautiful, with peculiar shape. There are two silver-white stomatal belts on the back of the leaves. When the breeze blows, the silver light is dazzling and has a unique style. Three-pointed fir wood is solid, with straight grain and compact structure, suitable for high-end furniture and interior decoration. Its seeds have a high oil yield and can be used in industry. In addition, the roots, stems, leaves, bark, and fruits of Cedarwood contain a variety of alkaloids, especially harringtonine and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何官榕何碧珠
Owner FUJIAN AGRI & FORESTRY UNIV
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