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Method for degrading crude oil with bacillus

A technology based on bacillus and crude oil, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of difficult removal of crude oil pollution, crude oil leakage and pollution, environmental and ecological damage, etc., to achieve Low cost, good effect, and low degradation cost

Inactive Publication Date: 2009-12-23
NINGBO UNIV
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  • Application Information

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Problems solved by technology

[0002] With the development of international economy and trade, the demand and transportation volume of crude oil are increasing. At the same time, there are more and more incidents of crude oil leakage and pollution. Crude oil pollution has caused great damage to the environment and ecology, and it will cost a lot to clean up crude oil pollution. The cost and long time, especially the difficulty of removing crude oil pollution in the ocean

Method used

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  • Method for degrading crude oil with bacillus
  • Method for degrading crude oil with bacillus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Set the concentration to 10 7 ~10 8 1ml Bacillus licheniformis bacteria solution per ml was inoculated into 100ml crude oil culture solution with a mass concentration of about 2% to prepare a degradation solution, and the degradation solution was degraded for 10 days at a temperature of 30-37°C and a shaking condition of 180r / min , using an infrared spectrometer to detect the degraded solution for 10 days, and the near-infrared spectrum is obtained as figure 1 As shown in c, the mid-infrared spectrum is shown in figure 2 shown in b.

Embodiment 2

[0022] Set the concentration to 10 7 ~10 8 Inoculate 1ml Bacillus licheniformis bacteria solution per ml into 100ml crude oil culture solution with a mass concentration of about 2% to prepare a degradation solution, and degrade the degradation solution for 15 days at a temperature of 30-37°C and a shaking condition of 180r / min , using an infrared spectrometer to detect the degraded solution for 15 days, and the near-infrared spectrum is obtained as figure 1 d as shown in

Embodiment 3

[0024] Set the concentration to 10 7 ~10 8 Inoculate 1ml Bacillus licheniformis bacteria solution per ml into 100ml crude oil culture solution with a mass concentration of about 2% to prepare a degradation solution, and degrade the degradation solution for 20 days at a temperature of 30-37°C and a shaking condition of 180r / min , using an infrared spectrometer to detect the degradation solution degraded for 20 days, the near-infrared spectrum is obtained as figure 1 e as shown in

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Abstract

The invention discloses a method for degrading crude oil with bacillus. Degradation solution is prepared according to the ratio of 80 to 150 ml of crude oil culture solution to 0.8 to 1.5 ml of bacillus solution, the degradation solution is degraded for 10 to 30 days with the temperature being 30 to 37 DEG C and the shaking condition being 150 to 200 r / min, the concentration of the bacillus solution is 10 to 10 bacilli / ml, and the mass concentration of the crude oil culture solution is 0.5 to 5 percent; the method adopts single type of bacillus, the trouble that the prior degradation bacillus solution is mainly prepared by two or more cultures is avoided, the operation is more convenient, additional nutrient solution is not required to be prepared, additional surface active agent is not required to be prepared either, the degradation cost can be lower, the effect of the method on crude oil degradation is good, and crude oil can be totally degraded in about 20 days. The invention is a method for degrading crude oil with bacillus, with the advantages of convenient operation, lower cost and better degradation effect.

Description

technical field [0001] The invention relates to crude oil degradation, in particular to a method for degrading crude oil with bacillus. Background technique [0002] With the development of international economy and trade, the demand and transportation volume of crude oil are increasing. At the same time, there are more and more incidents of crude oil leakage and pollution. Crude oil pollution has caused great damage to the environment and ecology, and it will cost a lot to clean up crude oil pollution. The cost and long time, especially the difficulty of removing crude oil pollution in the ocean. Scientists from various countries are actively studying the use of various methods (chemical methods, biological methods) to remove crude oil pollution. Chemical methods will cause secondary pollution. At present, the method that is widely concerned and economical is to use microorganisms to degrade crude oil, such as Chinese patent application No. 200710060178.5, the name As a me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02C02F3/34C12S1/00A62D101/20C12R1/07C12R1/10C12R1/125C02F101/32
Inventor 苏秀榕李妍妍李晔
Owner NINGBO UNIV
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