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Bivalent inactivated vaccine against gosling plague and goose paramyxovirus disease and preparation method thereof

A dual inactivated vaccine and goose paramyxovirus technology, which is applied in antiviral agents, virus antigen components, and pharmaceutical formulations, can solve the problems of less research, poor immune effect, and danger of goslings, and achieve simple emulsification procedures, The effect of suitable viscosity

Inactive Publication Date: 2011-07-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, gosling plague prevention mainly uses gosling plague attenuated vaccine to immunize goslings, but since sufficient protective antibodies can be produced in 7 to 10 days, this blank period is quite dangerous for highly exposed goslings, and production practice has also proved The immune effect is very poor, so there is an urgent need for a virulent inactivated vaccine for breeding geese to obtain maternal antibody protection for goslings
[0004] Goose paramyxovirus disease has not been reported abroad, and there are few domestic studies. Neither the inactivated vaccine nor the attenuated vaccine is blank.

Method used

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  • Bivalent inactivated vaccine against gosling plague and goose paramyxovirus disease and preparation method thereof
  • Bivalent inactivated vaccine against gosling plague and goose paramyxovirus disease and preparation method thereof
  • Bivalent inactivated vaccine against gosling plague and goose paramyxovirus disease and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the acquisition of goose plague and goose paramyxovirus disease dual inactivated vaccine

[0027] In order to simultaneously and safely prevent these two viral infectious diseases in goslings, a dual inactivated vaccine was developed. Its preparation method is as follows:

[0028] 1. Preparation of Gosling Plague Inactivated Vaccine

[0029] 1. Materials

[0030] Virus species: goose plague virus duck embryo attenuated GD strain (purchased from China Veterinary Drug Control Institute) E 20-7 To replace poison. The method for measuring the virus content of GPVGD strain is: serially dilute the virus seeds 10 times, and take 10 -4 、10 -5 、10 -6 3 dilutions, inoculate 5 8-10-day-old duck embryos in the allantoic cavity of each dilution, 0.3 ml per embryo, continue incubation at 37-37.5 degrees Celsius, and observe for 72-124 hours (duck embryos that died within this time are recorded as the calculated ELD 50 The death situation was recorded, the skin o...

Embodiment 2

[0123] Embodiment 2, the effect detection of goose plague virus disease, goose paramyxovirus disease dual oil emulsion inactivated vaccine

[0124] 1. Field test report of dual inactivated vaccine against goose plague and goose paramyxovirus disease

[0125] After completing the laboratory test of the dual inactivated seedlings, a field test was carried out with four batches of qualified laboratory products to observe the safety and efficacy of the dual seedlings under production conditions.

[0126] 1 material

[0127] Vaccine: the batch numbers 00201, 00202, 00203, and 00301 prepared in Example 1 are dual inactivated vaccines for gosling plague and goose paramyxovirus disease.

[0128] Immune breeding geese: select 4 test sites:

[0129] Chifeng Laiyinte Goose Industry Co., Ltd. 500

[0130] Baicheng Animal Husbandry and Veterinary Work Station 500

[0131] Meihekou City Animal Husbandry and Veterinary Work Station 1000

[0132] 1000 animal husbandry and veterinary work...

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Abstract

The invention discloses a bivalent inactivated vaccine against gosling plague and goose paramyxovirus disease and a preparation method thereof. The bivalent inactivated vaccine comprises an inactivated vaccine against gosling plague and an inactivated vaccine against goose paramyxovirus disease. The inactivated vaccine against gosling plague is obtained by inactivating blastochyle obtained by a bird embryo inoculating a duck embryonization lentogen strain of gosling plague; the inactivated vaccine against goose paramyxovirus disease is obtained by inactivating blastochyle obtained by a bird embryo inoculating a velogen strain of goose paramyxovirus. The method comprises: hatching a bird embryo of the duck embryonization lentogen strain inoculating a gosling plagues and a bird embryo of the velogen strain inoculating goose paramyxovirus; selecting embryos which die when hatched between 96 and 216 hours; collecting blastochyle; adding formaldehyde in the blastochyle and inactivation toobtain bivalent inactivated vaccine against gosling plagues and goose paramyxovirus disease.

Description

technical field [0001] The invention relates to a dual inactivated vaccine of goose plague and goose paramyxovirus disease and a preparation method thereof. Background technique [0002] Gosling plague (GD) is an acute and subacute sepsis caused by goose parvovirus (GPV). The disease mainly affects goslings 3 to 20 days after hatching. Viral disease (GPM) is a viral infectious disease caused by goose paramyxovirus (GPMV) with respiratory disorder and diarrhea as the main symptoms, which is very harmful to goslings. [0003] At present, gosling plague prevention mainly uses gosling plague attenuated vaccine to immunize goslings, but since sufficient protective antibodies can be produced in 7 to 10 days, this blank period is quite dangerous for highly exposed goslings, and production practice has also proved The immune effect is very poor, so there is an urgent need for a strong and inactivated vaccine for immunizing breeding geese so that goslings can obtain maternal antibod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P31/14A61K39/17A61K39/295A61P31/20A61K39/12
CPCY02A50/30
Inventor 丁壮丛彦龙母连志毕玉海
Owner JILIN UNIV
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