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Microbial synthesis method of gamma-linolenic acid oil

A technology for microbial synthesis and linolenic acid, applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve problems such as large-scale industrial production that has not yet been formed, and achieve stable industrial production performance, fast growth, and cost reduction. Effect

Inactive Publication Date: 2009-06-03
北京有容建业科技发展有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of them are still in the stage of laboratory research and have not yet formed large-scale industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1). Maintenance and activation of the original strain: inoculate the 108.16 strain of Mucor circiniferi into the slant medium, and cultivate it at 25° C. for four to five days.

[0060] (2). Liquid culture: Inoculate the activated strains in 500ml of liquid culture medium for culture, the culture vessel is a 1L culture bottle with magnetic stirring, the stirring speed is 120 rpm, the temperature is 28-30°C, the time for 18 hours.

[0061] (3). Expanded cultivation: the liquid strain is inoculated into a 5L laboratory fermenter with a ratio of 10% for cultivation. The ventilation rate is 1.2 L / min, the stirring is 420 rpm, the pH is 5.5-6.0, the temperature is 30°C, and the time is 18-24 hours.

[0062] (4). One-stage fermentation: add the strains of the expanded culture to a 50-500L fermenter with an inoculum of 10%, the ventilation rate is 1.2L / min, the stirring is 420 rpm, and the pH is 5.5-6.0. The temperature is 30° C., and the time is 18-24 hours. Observed with...

Embodiment 2

[0074] (1). Maintenance and activation of the original strain: inoculate the 108.16 strain of Mucor circiniferi into the slant medium, and cultivate it at 25° C. for four to five days.

[0075] (2).Liquid culture: Inoculate the activated strains into 500ml of liquid culture medium for culture, the culture vessel is a 1L culture bottle with magnetic stirring, the stirring speed is 125 rpm, the temperature is 28-30°C, the time for 19 hours.

[0076] (3). Expanded cultivation: the liquid strain is inoculated into a 5L laboratory fermenter with a ratio of 10% for cultivation. The ventilation rate is 1.3 L / min, the stirring is 450 rpm, the pH is 6.0-6.5, the temperature is 30°C, and the time is 18-24 hours.

[0077] (4). One-stage fermentation: add the strains of the expanded culture to a 50-500L fermenter with an inoculum of 10%, the ventilation rate is 1.3L / min, the stirring is 450 rpm, and the pH is 6.0-6.5. The temperature is 30° C., and the time is 18-24 hours. Observed wit...

Embodiment 3

[0089] (1) Maintenance and activation of the original strains: inoculate the 108.16 strain of Mucor circiniferae on the slant medium, and culture at 25° C. for four to five days.

[0090] (2) Liquid culture: Inoculate the activated strains into 500ml of liquid culture medium for culture, the culture vessel is a 1L culture bottle with magnetic stirring, the stirring speed is 130 rpm, the temperature is 28-30°C, and the time is 20 hours.

[0091] (3) Expanded cultivation: the liquid bacteria were inoculated into a 5L laboratory fermenter with a ratio of 10% for cultivation. The ventilation rate is 1.5 L / min, the stirring is 480 rpm, the pH is 5.8-6.2, the temperature is 30°C, and the time is 18-24 hours.

[0092] (4) Primary fermentation: the bacterial classification that expands culture is added in the fermentation tank of 50-500L with 10% inoculum, ventilation rate is 1.5L / min, stirring 480 rpm, pH is 5.8-6.2, temperature For this purpose 30°C for 18-24 hours. Observed with...

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Abstract

A method for producing gamma-linolenic acid oil by microbial fermentation comprises the following process steps: culturing high-level expression strains of malic enzyme and gamma-linolenic acid synthetase; performing activation and amplification culture on original strains; then performing primary fermentation, secondary fermentation, third-stage fermentation and a fourth-stage fermentation; dehydrating, drying and extracting the oil. The method has the advantages of good strains, high yield, high dry bacteria yield up to 10-30%, good oil yield up to 35-55%, satisfactory gamma-linolenic acid yield up to 18-29% and low production cost. The method can help reduce the cost of raw materials for the production process by 50-70%. The semi-continuous fermentation or continuous fermentation process is advanced, scientific and mature, can be used for large scale industrialized production, and achieve 50-500t fourth-stage fermentation level.

Description

1. Technical field: [0001] The invention relates to a preparation method of GLA oil, in particular to a method for biosynthesizing GLA oil by using a microbial fermentation method. Two background technology: [0002] The chemical name of GLA (γ-Linolenic acid) is octadecatrienoic acid, which is an isomer of α-linolenic acid. In a sense, it is one of the essential fatty acids in the human body. It undergoes complex metabolic processes in the body , produce a variety of secondary metabolites, mainly forming dihomo-gamma-linolenic acid (DHGL) or arachidonic acid (AA), which are then converted into prostaglandins (P), leukotrienes (I) and thromboxane (T). Since GLA and its series of metabolites have important and extensive physiological functions in the immune system, cardiovascular system, reproductive system, and endocrine system, its potential medicinal value is reflected in the following aspects: [0003] The growth inhibitory effect of GLA on various Gram-negative bacteri...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
Inventor 宋元达
Owner 北京有容建业科技发展有限责任公司
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