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Biological preparation capable of preventing and treating cruciferae club root and use thereof

A cruciferous and biological preparation technology, applied in the field of microorganisms, can solve problems such as clubroot disease of cruciferous family that have not yet been seen

Inactive Publication Date: 2011-08-17
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, so far, there have been no reports in the world of using microbial biocontrol agents to successfully control cruciferous clubroot in fields.

Method used

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  • Biological preparation capable of preventing and treating cruciferae club root and use thereof
  • Biological preparation capable of preventing and treating cruciferae club root and use thereof
  • Biological preparation capable of preventing and treating cruciferae club root and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Bacillus subtilis (Bacillus subtilis) XF-1 was transferred to a test tube slant medium, and the formula for test tube slant culture was: 1% tryptone, 0.5% yeast extract, 1% NaCl, 1.5% agar, pH7.0. Cultivate in an incubator at 30°C for 2 days to obtain test tube species.

[0068] Liquid fermentation medium formula: soybean flour 2.0%, corn flour 1.0%, glucose 0.3%, peptone 0.3%, CaCO 3 0.2%, (NH 4 ) 2 SO 4 0.05%, MgSO 4 .7H 2 O0.03%, KH 2 PO 4 0.1%, NaOH 0.1%, peanut oil 0.2%, defoamer 0.01%, pH6.8-7.0; inoculate the test tube into 500ml Erlenmeyer flask (200ml per bottle) liquid medium, at 30°C, the rotation speed is 150rpm Cultivate on a shaker for 48 hours, and the resulting culture solution is the biological preparation of the present invention.

Embodiment 2

[0070]At first will obtain test tube seed (with embodiment one), test tube seed is inoculated in 500ml Erlenmeyer flask (every bottle adorns 200ml) liquid medium, medium formula is: soybean flour 2.0%, corn flour 1.0%, glucose 0.3%, peptone 0.3%, CaCO 3 0.2%, (NH 4 ) 2 SO 4 0.05%, MgSO 4 .7H 2 O0.03%, KH 2 PO 4 0.1%, NaOH 0.1%, peanut oil 0.2%, defoamer 0.01%, pH6.8-7.0. Cultivate at 30° C. for 48 hours on a shaker with a rotating speed of 180 rpm, and the obtained culture solution is the biological preparation of the present invention.

Embodiment 3

[0072] At first will obtain test tube seed (with embodiment one), test tube seed is inoculated in 500ml Erlenmeyer flask (every bottle adorns 200ml) liquid medium, medium formula is: soybean flour 2.0%, corn flour 1.0%, glucose 0.3%, peptone 0.3%, CaCO 3 0.2%, (NH 4 ) 2 SO 4 0.05%, MgSO 4 .7H 2 O0.03%, KH 2 PO 4 0.1%, NaOH 0.1%, peanut oil 0.2%, defoamer 0.01%, pH6.8-7.0. Cultivate at 30° C. for 48 hours on a shaker with a rotating speed of 200 rpm, transfer the obtained culture liquid to a fermenter for fermentation production, and obtain the fermented liquid which is the biological preparation of the present invention.

[0073] Production conditions in the fermenter:

[0074] Tank temperature: The temperature in the fermenter is controlled at 30°C to 37°C, measured by a thermometer inserted into the culture medium, and adjusted by passing cooling water or hot water into the interlayer.

[0075] Tank pressure: The tank pressure of the fermenter is controlled at 0.5 / s...

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Abstract

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony islight yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum;(2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and mainly relates to a biological agent for preventing and treating clubroot of cruciferous crops and its application. Background technique [0002] Clubroot was first discovered in the west coast of the Mediterranean Sea and southern Europe. At present, it is distributed in all countries in the world, causing at least 10% of economic losses. Clubroot is also widely distributed in Zhejiang, Guangdong, Guangxi, Hunan, Fujian, Jiangxi, Jiangsu, Anhui, Sichuan, Yunnan, Xinjiang, Tibet, Liaoning, Shandong, Shanghai, Beijing and Chongqing and other provinces (cities and autonomous regions), It can be said that it basically covers the main planting area of ​​cruciferous plants in our country. The disease is caused by a lower and more primitive fungus --- Plasmodiophora brassicae (or known as protozoa), which is extremely difficult to control. The fungus is an obligate parasite, which mainly h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P3/00C12N1/20C12R1/125
Inventor 何月秋熊国如范成明
Owner YUNNAN AGRICULTURAL UNIVERSITY
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