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Uses of MRPINK in restraining liveness of Macrobracium rosenbergii spermatozoon

A technology of Macrobrachium rosenbergii and sperm is applied in the application field of MRPINK in inhibiting sperm activity of Macrobrachium rosenbergii, which can solve the problems of zero awareness and operability.

Inactive Publication Date: 2011-05-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current understanding of this aspect is only limited to the morphological structure, general histochemical, biochemical components and basic physiological indicators, and the understanding and operability at the gene level are almost zero.

Method used

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  • Uses of MRPINK in restraining liveness of Macrobracium rosenbergii spermatozoon
  • Uses of MRPINK in restraining liveness of Macrobracium rosenbergii spermatozoon
  • Uses of MRPINK in restraining liveness of Macrobracium rosenbergii spermatozoon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Obtaining of MRPINK gene and its encoded protein

[0018] For details, please refer to the literature: Cao JX, Dai JQ, Dai ZM, Yin GL and Yang WJ. Amale-reproduction-related kazal-type peptide inhibitor gene in the prawn, Macrobrachium rosenbergii: molecular characterization and expression patterns. Marine Biotechnology. 2007, 9: 45~55.

[0019] ① Take the male and female reproductive system tissues of Macrobrachium rosenbergii, and extract total RNA according to the instructions of the Trizol kit;

[0020] ②Establish the SSH cDNA library of the male reproductive system of Macrobrachium rosenbergii, and screen the differentially expressed gene fragments:

[0021] A. Synthesis of the first strand: Extract PolyA according to Oligotex mRNA Kits method + RNA to PolyA + RNA is used as a template for reverse transcription to synthesize single-stranded cDNA.

[0022] B. Synthesis of double-stranded (ds cDNA), digested with Rsa I enzyme to generate blunt ends.

...

Embodiment 2

[0054] Example 2: Inhibitory effect of MRPINK on the sperm activity of Macrobrachium rosenbergii

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Abstract

The invention provides application of the specificity Kazal type protease inhibitor MRPINK of the male genital system of the giant freshwater shrimp in inhibiting the germ cell activity of the giant freshwater shrimp. By research and adding MRPINK in the germ cell of the giant freshwater shrimp, the germ cell activity can be effectively inhibited, and the egg can not be fertilized; and the similar protease inhibitor is added in the frozen germ cell, the endoenzyme activity of the germ cell is inhibited, and the germ cell is inactivated, so that the invention has important referential meaning for the low-temperature storage of the germ cell.

Description

(1) Technical field [0001] The invention relates to the application of MRPINK, a specific Kazal-type protease inhibitor of Macrobrachium rosenbergii, in inhibiting sperm activity of Macrobrachium rosenbergii. (2) Background technology [0002] Biological sex and reproductive phenomena are one of the main contents of biological research, which involves many important biological issues, such as sex determination, heredity, development and evolution, so it has always attracted much attention. At present, studies on the molecular mechanisms of sex and reproduction are mainly concentrated in humans and model organisms, while there are few studies on general aquatic organisms, especially in crustaceans, where the known molecular information is even more scarce. [0003] Shrimp, as an aquatic organism with important economic value, has relatively little research at the molecular level. Therefore, it is particularly necessary to obtain a large number of genetic resources related to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/55A61K35/52C12N5/08A61P15/16
Inventor 马文明曹俊霞杨卫军
Owner ZHEJIANG UNIV
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