System for performing intra-operative angiography
A surgical, cardiovascular technique applied in the field of blood flow in the cardiovascular system
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[0098] The described examples show the use of a preferred device of the present invention in observing the flow of fluorescent dye through a specific vessel, i.e. the mouse femoral artery, and the Langendorff perfused heart, and also show that the device works under normal conditions and under the influence of locally applied acetylcholine The ability to determine the diameter of mouse femoral artery vessels.
[0099] In this example, a fluorescent dye (ICG) is injected into the vascular bed (via a mouse jugular catheter: a perfusion catheter that perfuses the heart via a langendorff) and excited with the original radiation (806 nm) from a laser. Fluorescence (radiation) emitted by the dye (830 nm) is captured with a CCD camera as a series of vascular imaging images. The angiographic image relayed by the camera becomes the analog-to-digital conversion software running on the PC to digitize the angiographic image. These digitized images are then analyzed qualitatively (by view...
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