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Method for synthesizing polymyxin B by fermentation method

A technology of polymyxin and fermentation method is applied in the field of biosynthesis of polymyxin B by fermentation method, which can solve the problems of single nitrogen source, low content and low yield, etc., and achieves the effect of increasing the fermentation unit

Inactive Publication Date: 2008-08-06
SHANGHAI DUOMIRUI BIOLOGICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the comparative literature The Biosynthesis of Polymyxin B by Growing Cultures of Bacillus polyrnyxa also discloses a culture medium for biosynthesizi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Prepare culture medium according to the following formula, wherein culture medium a is the formula of the present invention, and culture medium b is the formula of comparative literature:

[0017] Medium a: 60g soybean protein isolate, 30g starch, 1g ammonium sulfate, CaCO 3 8g, MgSO 4 ·7H 2 O 0.02g, MnSO 4 ·H 2 O 0.01g, distilled water to 1L.

[0018] Medium b: Glucose 5g (single elimination at a concentration of 50%), (NH 4 ) 2 SO 4 1.5g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.1g, FeSO 4 ·7H 2 O 0.01g, ZnSO 4 0.01g, MnSO 4 ·H 2 O0.0075g, 40ml 0.5M K 3 PO 4 , biotin 0.5 μg.

[0019] The medium a and b were both inserted into the seed culture of bacillus polymyxa ATCC 10401, the inoculum size was 7%, and the pH value before disinfection was adjusted to 7.2; the temperature was 30°C; the filling volume was 30ml / 250ml; after 40 hours of cultivation, the bottle was placed, and the result of the former was 6534.5 U / ml polymyxin B was obtained, which gave 1043 U / ml pol...

Embodiment 2

[0021] 60 g of soybean protein isolate in medium a was replaced with 40 g of soybean protein isolate, resulting in 5652.1 U / ml polymyxin B.

Embodiment 3

[0023] 60 g of soybean protein isolate in culture medium a was replaced with 100 g of soybean protein isolate, and as a result, 6072.3 U / ml polymyxin B was obtained.

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Abstract

The invention discloses a method for synthesizing polymyxin B through a fermentation method, which comprises: inoculating bacillus polymyxa ATCC10401 into culture medium to culture and is characterized in that the concentration of the organic nitrogen source which is used in the culture medium is 4%-10%. The highest fermentation unit of the polymyxin B which is synthesized through fermenting by the method can reach 6534.7U/ml.

Description

technical field [0001] The invention relates to a method for biosynthesizing polymyxin B by a fermentation method. Background technique [0002] Polymyxin is a general term for a family of basic cyclic polypeptide antibiotics composed of various amino acids and fatty acids produced by Bacillus polymyxa. So far, it has been found that among multiple components of polymyxin A, B, C, D, E, M, etc., only polymyxin B (polymyxinB, polymyxin for short), polymyxin E (polymyxinE, Colistin for short) ) has low toxicity and good antibacterial activity. Therefore, there are relatively many studies on polymyxin B and polymyxin E, and the corresponding products have been used clinically. Their sulfate or methanesulfonate are commonly used clinically, and the latter is mostly used for parenteral administration, and polymyxin is hydrolyzed in tissues to play a role. It can be used to treat some meningitis and dysentery caused by Gram-negative bacteria, especially it can inhibit Pseudomon...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12R1/12
Inventor 齐艳艳陈雪田永辉赵文杰冯军程睛华林纲薛春佳朱裕辉魏晓东
Owner SHANGHAI DUOMIRUI BIOLOGICAL TECH CO LTD
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