Turf grass stunt related gene GA20

Abstract

The invention clones GA20 oxydase gene from an excellent grass seed for lawn-Korea lawn grass and constructs the plant expression vector for the grass seed. By degenerating PCR and RACE method, GA20 oxydase gene is cloned from wild Korea lawn grass of China. The total length of the gene is 1311 bp, the code area length is 1109 bp, the code area is 96-1205 bp including 369 amino acid. The gene is of 65% homology with wheat and rice. The login no. on GenBank is DQ645453. In addition, the invention constructs the positive and reverse expression vector for the GA20 oxydase gene based on pC1303. The vector contains GUS report gene and hygromix marking gene, can be used for seed breeding in gene engineering of lawn grass so as to culture short transgenosis lawn grass.

Description

1. Technical field: [0001] The invention belongs to the field of plant biotechnology or the field of crop breeding. Specifically, related technologies related to the cultivation of Zoysia weed, RNA extraction, primer design, gene cloning and vector construction. Thus, the GA20 oxidase gene was obtained from Zoysia pratense, and the sense and antisense plant expression vectors of the gene were constructed. Two background technologies: [0002] The lawn has the functions of greening and landscaping, water and soil conservation, microclimate adjustment, viewing and sports, etc. It plays a decisive role in residents' rest, entertainment and landscape. At present, more than 4,000 hectares of lawns have been planted in Beijing, with an annual increase of more than 250 hectares, which has played an important role in beautifying Beijing's environment and improving the quality of life of the people in the capital. Yet lawn pruning and irrigation etc. expend a large amount of manpow...

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Problems solved by technology

However, there is no report on this aspect in combination with gibberellin synthesis-related genes.

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