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Bifenthrin antigen, antibody and uses thereof

A technology of bifenthrin and antigen, applied in the field of immunochemical analysis, achieves the effect of simple method

Inactive Publication Date: 2011-06-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the immunoassay method of bifenthrin has not been reported at home and abroad.

Method used

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  • Bifenthrin antigen, antibody and uses thereof
  • Bifenthrin antigen, antibody and uses thereof
  • Bifenthrin antigen, antibody and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] 1 Synthesis of Bifenthrin Artificial Hapten

[0033] Based on the fact that 2-methyl-3-phenylbenzyl alcohol is the main component in the molecular structure of bifenthrin, and can fully expose the structural features and contain carboxyl groups that can be coupled with proteins, 2-methyl- Using 3-phenylbenzyl alcohol, succinic anhydride and anhydrous pyridine as raw materials, the hapten 2-methyl-3-phenylbenzylsuccinate (LBc for short) was synthesized through a one-step reaction. In order to improve the detection sensitivity through heterologous reaction, the hapten 2-methyl-3- Phenylbenzoic acid (abbreviated as LBy). After purification, the products LBc and LBy were subjected to mass spectrometry (ESI) and H NMR spectroscopy ( 1 H-NMR) identification, and confirmed by comparison with similar known compound data.

[0034] The structure of the hapten is as follows:

[0035] or

[0036] 2-Methyl-3-phenylbenzylsuccinate (LBc) 2-Methyl-3-phenylbenzoic acid (LBy)

...

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Abstract

The method comprises: synthesizing the hapten 2-methyl-3-phenyl benzyl succinic acid ester (for short: LBC) and the 2-methyl-3-phenylbenzene benzoic acid (for short: LBY); coupling the LBc with protein to prepare the artificial immunity antigen and immunity rabbit to the specificity polyclonal antibody of Bifenthrin; coupling the LBy with the protein to prepare the envelope antigen; using the synthesized envelope antigen and specificity antibody to establish the enzyme immunoassay EIA analysis method; the detection range of the Bifenthrin can reach 0.016mg / L, and the linear range of it is 0.01-50mg / kg.

Description

1. Technical field [0001] The invention relates to a bifenthrin antigen, an antibody and applications thereof, belonging to the technical field of immunochemical analysis, and is specially used for the preparation of bifenthrin-specific antibodies and an enzyme-linked immunoassay method. 2. Background technology [0002] Bifenthrin (bifenthrin), trade name Uranus, in 1978 FMC Corporation (FMC) of the United States published a synthesis patent, registered in China in 1988, the chemical name and structure are shown in the figure below, the pure product is an off-white body mp: 68~ At 70°C, the solubility in water is 0.1mg / L (25°C), and it is stable under acidic conditions. It is a pyrethroid insecticide and acaricide, which has stomach poisoning and contact killing effects, and has a long lasting effect. It can be used to control crop pests, household termites, woolworms, etc. It is the only one among pyrethroid pesticides that can be used to control tea garden pests. medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53C07C69/40C07C63/331
Inventor 王鸣华施海燕李波
Owner NANJING AGRICULTURAL UNIVERSITY
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