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Producing method for double-layer artificial nerve catheter

An artificial nerve and catheter technology, which is applied to the human body tubular structure device, catheter, medical science and other directions, can solve the problem of no artificial nerve catheter, etc., and achieve the effect of fast regeneration, good elasticity and strength, and good quality.

Inactive Publication Date: 2009-04-22
首都医科大学北京神经科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the biocompatibility and mechanical properties of the catheter, there is no ideal artificial nerve catheter for spinal cord regeneration.

Method used

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  • Producing method for double-layer artificial nerve catheter
  • Producing method for double-layer artificial nerve catheter
  • Producing method for double-layer artificial nerve catheter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, preparation double-layer artificial nerve guide

[0023] In this embodiment, the outer catheter is prepared with chitosan, and the inner catheter is prepared with collagen, and then the inner catheter is inserted into the outer catheter, and finally, the fibrin gel is injected into the gap of the double-layer catheter, that is A double-layer artificial nerve guide was prepared.

[0024] Wherein, the preparation method of chitosan conduit is:

[0025] 1. Dissolve chitosan in acetic acid solution;

[0026] 2. Pour the chitosan solution into the core material mold for molding, and perform thermal dehydration and cross-linking treatment;

[0027] 3. Repeatedly dipping and air-drying the obtained catheter in the chitosan solution for several times.

[0028] Collagen conduits are prepared by:

[0029] 1. Dissolve collagen in hydrochloric acid solution;

[0030] 2. Pour the collagen solution into the core material mold for molding, and freeze-dry it;

[003...

Embodiment 2

[0038] Embodiment 2, double-layer artificial catheter is used for the repair of sheep sciatic nerve

[0039] A double-layer artificial nerve guide was prepared according to the method in Example 1, wherein 100 ng of nerve growth factor NGF was added to each milliliter of fibrin glue.

[0040] Remove 10 cm of the common peroneal nerve from a sheep (20 kg in weight), insert the stumps of the nerves on both sides into the above-mentioned catheter, suture the overlapping part of the catheter and the nerve with 10-0 nylon thread, observe dynamically for 1 year, and conduct morphological examination , electrophysiological and behavioral evaluations.

[0041] The results of the study showed that the animal's behavior disorder began to recover at the 3rd month after the transplant operation, there was no obvious movement disorder at the 6th month, and it returned to normal at the 12th month without weight bearing. WGA-HRP was used for nerve tracing and Neurfilament (NF) immunohistoch...

Embodiment 3

[0042] Embodiment 3, double-layer artificial catheter is used for the repair of rat spinal cord tissue

[0043] According to the method of Example 1, a catheter with a length of 5 mm and an outer diameter of 2.5 mm was prepared.

[0044]Take adult Wistar rat spinal cord transection as a model: perform conventional surgery, open the laminae, completely resect the thoracic 9-10 segment of the rat spinal cord by 5 mm, transplant the above-mentioned catheter to the injured part, and suture the dura mater. Dynamic observation for 1 year, and evaluation of morphology, electrophysiology and BBB21 behavior.

[0045] The results of the study showed that BDA was used for antegrade nerve tracing, Neurfilament (NF) immunohistochemical staining, Gless silver-plated staining, etc. all proved the reconstruction of morphological structure; a large number of myelinated and unmyelinated nerve fibers were observed by electron microscope ; In the study of electrophysiology, it also proved the re...

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Abstract

The invention discloses an artificial nerve conduit of double layer and the method for preparing same. The said nerve conduit comprises an inner conduit and an outer layer, which are made from degradable and adsorptive material in living body. The slit between said inner conduit and outer conduit is filled with fiber albumen glue (used for regeneration of nervous tissue and blood vessel). The artificial nerve conduit of double layer can be used for bypass surgery for damaged round nerve and spinal marrow, and it will be degraded and adsorbed in living body after nerve regeneration. The said artificial nerve conduit possesses good elasticity and strength, and the degradation time is adaptive to growing speed of nervous fiber (about 1-6 months).

Description

technical field [0001] The invention relates to a double-layer artificial nerve guide and a preparation method thereof. Background technique [0002] When peripheral nerves are damaged or defective, the first method people choose is to directly anastomose the stumps of the nerves on both sides. If it cannot be directly anastomosed, due to the obstacles of the surrounding connective tissue, etc., the new nerve fibers will not be able to reach the target tissue through the defect site, and a proliferative neuroma-like tissue will be formed at the stump of the regenerated nerve, resulting in Dysfunction of the extremity where the trauma occurred. In addition, autologous nerve transplantation is also used to repair peripheral nerve damage. However, using this method will not only cause functional defects in the part where the material is obtained, but also the functional recovery of the part where the nerve is transplanted is not satisfactory, and the allogeneic nerve transpla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/04A61L27/24A61L27/58A61L29/04A61L29/14
Inventor 李晓光杨朝阳
Owner 首都医科大学北京神经科学研究所
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