Process for preparing copolyester soybean esterase
A technology of soybean and esterase, which is applied in the direction of hydrolytic enzymes, etc., can solve the problems of detection sensitivity of residual pesticides and the minimum detection limit failing to meet the safety limit requirements, etc.
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[0006] Concrete operation steps of the present invention are as follows:
[0007] a. Choose high-quality soybeans,
[0008] b. Grinding into fine powder with a pulverizer,
[0009] c. Phosphate buffer extraction,
[0010] c.1 Put the crushed soybean flour into a container of appropriate size, and add 0.3mol / L, pH 7.0-8.0 phosphate buffer solution at a ratio of 1:4-5 to liquid;
[0011] c.2 Shake or stir on a shaker or mixer for 10-30 minutes;
[0012] c.3 After shaking or stirring, continue to stand and extract in the above container
[0013] c.3.1 The temperature is 0-4°C
[0014] c.3.2 The time is 10-24h;
[0015] c.4 Filter the above extract with gauze, and take the supernatant, which is the crude extract;
[0016] d. Differential refrigerated centrifugation,
[0017] d.1 Centrifuge the crude extract at 5000-6000rpm for 10-15min, and collect the supernatant;
[0018] d.2 Dissolve the precipitate in the previous step with phosphate buffer solution of pH 7.0-8.0, then...
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