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Production method of insect specimen

A production method and insect technology, applied in the preservation of human or animal bodies, instruments, teaching models, etc., can solve the problems of specimen loss of color, tissue and cell structure damage, specimen deformation, etc., and achieve the effect of avoiding polymerization damage

Inactive Publication Date: 2008-10-01
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although soaked specimens can effectively preserve the whole worm, the specimens will soon lose their original color; dried specimens, although the original color of the specimens can be maintained for a period of time, the specimens are more or less stained. Some are deformed and easily eaten by moths; specimens embedded in plexiglass, rosin and urea-formaldehyde resin are mainly embedded with chitin shells and small insects. These insects are characterized by natural drying, and the surface of the insects is not It will be deformed, but the tissue and cell structure in the body have been destroyed, so it can only be viewed and cannot be used for further structural research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method steps are as follows:

[0030] 1. Fixation: live insects were placed in 7% glutaraldehyde fixative solution for 60 minutes.

[0031] 2. Gradual dehydration with ethanol: plasticize the fixed insects, soak in ethanol at concentrations of 35%, 50%, 75%, 85%, 95%, and 100% for 30-60 minutes in each gradient.

[0032] 3. Replacement: Pass the insect specimens in 100% ethanol through the mixture of ethanol and xylene, wherein the volume ratio of xylene is 35%, 50%, 75%, 85%, 95%, 100%, each gradient replacement 30-60 minutes;

[0033] 4. Infiltration: through the mixture of xylene and neutral gum, in which the volume ratio of neutral gum is 30% for 1 hour, 60% for 3 times, each time for 1 hour;

[0034] 5. Solidification: Place the insects in 60% neutral gum by volume in step 4 overnight, then remove, remove excess gum, and let it dry.

Embodiment 2

[0036] The method steps are as follows:

[0037] 1. Fixing: same as embodiment 1.

[0038] 2. Stepwise dehydration of ethanol: same as in Example 1.

[0039] 3. Replacement: through a mixture of acetone and ethanol, wherein the volume ratio of acetone is 35%, 50%, 75%, 85%, 95%, 100%, and each gradient is 30-60 minutes.

[0040] 4. Penetration: The resin 618 penetrant formula used is:

[0041] Resin 618 DDSA DBP BMP-30

[0042] 30g 16.3g 2.5g 0.6g

[0043] Insects in acetone were placed in volume ratio Resin 618 Penetrant:Acetone-1:1 (room temperature, 3 hours), Resin 618 Penetrant:Acetone-3:1 (room temperature, 3 hours), Resin 618 Penetrant (room temperature, , 10 hours).

[0044] 5. Polymerization: Take out the insects in the resin 618 penetrant, remove the excess resin 618 penetrant, and place it at 35°C for 24 hours, at 45°C for 24 hours, and at 68°C for 48 hours.

Embodiment 3

[0045] Embodiment 3, method steps are as follows:

[0046] 1. Fixing: same as embodiment 1.

[0047] 2. Stepwise dehydration of ethanol: same as in Example 1.

[0048] 3. Replacement: with embodiment 2.

[0049] 4. Penetration: The formula for using Epon812 penetrant is:

[0050] Epon812 DDSA MNA DMP-30

[0051] 13ml 8ml 7ml 0.6ml

[0052] Place the insects in acetone in volume ratio Epon812 penetrant: acetone-1:1 (room temperature, infiltrate for 3 hours), Epon812 penetrant: acetone-3:1 (room temperature, infiltrate for 3 hours), Epon812 penetrant (room temperature, infiltration for 10 hours).

[0053] 5. Polymerization: with embodiment 2.

[0054] The insect specimens obtained in the above embodiments not only maintain the original color and shape of the insect specimens, but also are not rotten or moth-eaten, and become permanent preservation specimens.

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Abstract

The invention discloses a preparing method of insect sample, which comprises the following steps: fixing living insect, dehydrating through alcohol, replacing, permeating, solidifying or polymerizing to obtain the insect sample, fitting for studying microscopic structure and super-microscopic structure of insect cell.

Description

technical field [0001] The invention relates to a method for making animal specimens, in particular to a method for making insect specimens that can be permanently preserved. Background technique [0002] In order to preserve the insects for a long period of time, it is necessary to make specimens. The current insect specimens are mainly soaked specimens, dried specimens and embedded specimens, also known as amber specimens (the embedding agent is selected from plexiglass, rosin and urea-formaldehyde resin). Although soaked specimens can effectively preserve the whole worm, the specimens will soon lose their original color; dried specimens, although the original color of the specimens can be maintained for a period of time, the specimens are more or less stained. Some are deformed and easily eaten by moths; specimens embedded in plexiglass, rosin and urea-formaldehyde resin are mainly embedded with chitin shells and small insects. These insects are characterized by natural ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00G09B23/36
Inventor 张小民马恩波郭亚平李晓玲金晓弟
Owner SHANXI UNIV
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