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Diagnosis of diseases and conditions by analysis of histopathologically processed biological samples using liquid tissue preparations

a technology of liquid tissue preparation and which is applied in the field of diagnosis of diseases or conditions in subjects by analysis of biological samples, can solve the problems of lack of sensitivity, inability to provide quantitative, and limited number of assays that can be performed on formalin-fixed samples, and achieve the effect of increasing predisposition

Active Publication Date: 2009-08-27
EXPRESSION PATHOLOGY
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  • Abstract
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  • Claims
  • Application Information

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Benefits of technology

The invention provides a method for comparing protein analytes from a biological sample obtained from a subject with those of a known disease or condition to determine if the subject has an increased predisposition to develop the disease or condition. The analytes can be peptides derived from Her2, estrogen receptor, progesterone receptor, PSA, PAP, PEBP, GDF15, beta-amyloid protein, tau protein, alpha synuclein, parkin, UCH-L1, or NR4A2. The method can be used for cancer, Alzheimer's disease, Parkinson's disease, or benign hyperplastic condition.

Problems solved by technology

The technical problem addressed by this patent is the limitations caused by protein cross-links induced by formalin fixation, making it difficult to perform accurate and reliable diagnostic tests based on biological samples.

Method used

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  • Diagnosis of diseases and conditions by analysis of histopathologically processed biological samples using liquid tissue preparations
  • Diagnosis of diseases and conditions by analysis of histopathologically processed biological samples using liquid tissue preparations
  • Diagnosis of diseases and conditions by analysis of histopathologically processed biological samples using liquid tissue preparations

Examples

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example 1

Preclinical Diagnosis of Prostate Cancer Using an Antibody-Based Immunodetection Assay Analysis of Liquid Tissue Preparation

[0057]A plurality of Liquid Tissue preparations were prepared from a biological sample obtained from the prostate of a human subject. A first Liquid Tissue preparation was prepared from a region of the tissue containing prostate cancer (upper box), and a second Liquid Tissue preparation was prepared from a region of the tissue containing normal cells (lower box). The preparations were spotted onto a membrane, and differential levels of the protein Prostate Specific Antigen (PSA) were determined by a standard immunoassay. PSA is used in current clinical analyses as a diagnostic indicator of prostate cancer. As shown in the line graph of FIG. 2, a high level of PSA was detected in the cancer region was observed, whereas no detectable level of PSA was seen in the normal region of the tissue.

[0058]A plurality of Liquid Tissue preparations were prepared from a biolo...

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Abstract

The invention provides methods for diagnosing diseases such as cancer and other conditions using biological samples. Liquid Tissue samples prepared from histopathologically prepared tissue obtained from a subject surprisingly can be used to identify and, optionally, to quantify analytes that are diagnostic of the presence of a disease, condition or syndrome in the subject.

Description

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Claims

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Application Information

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Owner EXPRESSION PATHOLOGY
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