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Substituted piperazines

a technology of substituted piperazines and substituted piperazines, which is applied in the direction of biocide, drug composition, immunological disorders, etc., can solve the problems of inability to fully absorb the drug, short half-lives once administered, and prohibitive development and manufacturing costs, so as to achieve favorable solubility in water and increase the partitioning into selected solvents

Inactive Publication Date: 2008-10-23
CHEMOCENTRYX INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While function-blocking antibody and small peptide therapies are promising, they suffer from the perils of degradation, extremely short half-lives once administered, and prohibitive expense to develop and manufacture, characteristic of most proteins.

Method used

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  • Substituted piperazines
  • Substituted piperazines
  • Substituted piperazines

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0112]The piperazine ring can be formally attached to the terminal aryl unit in a number of ways: by aromatic nucleophilic displacement reactions, metal catalyzed coupling reactions (arylation reactions of secondary amines), ring expansion, rearrangement and cyclization reactions and the like. Also, different protection / deprotection strategies can be utilized. Hence, either all or only part of the final molecular architecture can be present during the key aryl coupling step. Examples for a variety of such aryl coupling strategies are listed below.

Protocol A

Metal Catalysed Arylation Reactions of Secondary Amines

Synthesis of (5-Chloro-2-piperazin-1-yl-phenyl)-phenyl-methanone

[0113]

[0114]Piperazine (3.6 g, 42.5 mmol), Pd(II)acetate (0.007 g, 0.043 mmol), sodium t-butoxide (0.22 g, 2.4 mmol) and BINAP (0.042 g, 0.068 mmol) were stirred at room temperature in 10 mL dry toluene for 15 min. (2-Bromo-5-chloro-phenyl)-phenyl-methanone (0.5 g, 1.7 mmol) in 10 mL dry toluene was then added int...

example 2

[0569]This example illustrates the activity associated with representative compounds of the invention.

[0570]Materials and Methods

A. Cells

[0571]CCR1 Expressing Cells

[0572]a. THP-1 Cells

[0573]THP-1 cells were obtained from ATCC and cultured as a suspension in RPMI-1640 medium supplemented with 2 mM L-glutamine, 1.5 g / L sodium bicarbonate, 4.5 g / L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 0.05% 2-mercaptoethanol and 10% FBS. Cells were grown under 5% CO2 / 95% air, 100% humidity at 37° C. and subcultured twice weekly at 1:5 and harvested at 1×106 cells / ml. THP-1 cells express CCR1 and can be used in CCR1 binding and functional assays.

[0574]b. Isolated Human Monocytes

[0575]Monocytes were isolated from human buffy coats using the Miltenyi bead isolation system (Miltenyi, Auburn, Calif.). Briefly, following a Ficoll gradient separation to isolate peripheral blood mononuclear cells, cells were washed with PBS and the red blood cells lysed using standard procedures. Remaining cells were lab...

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Abstract

Compounds are provided that act as potent antagonists of the CCR1 receptor, and which have been further confirmed in animal testing for inflammation, one of the hallmark disease states for CCR1. The compounds are generally aryl piperazine derivatives and are useful in pharmaceutical compositions, methods for the treatment of CCR1-mediated diseases, and as controls in assays for the identification of competitive CCR1 antagonists.

Description

CROSS-REFERENCES TO RELATED APPLICATIONS[0001]This application claims the benefit of Provisional Application Ser. No. 60 / 453,711, filed Jun. 12, 2002, (originally U.S. Ser. No. 10 / 171,398, filed Jun. 12, 2002) the contents of which is incorporated herein by reference.STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]This application was supported in part by DARPA Grant No. N65236-99-1-5420. The government of the United States may have certain rights in this application.REFERENCE TO A “SEQUENCE LISTING,” A TABLE, OR A COMPUTER PROGRAM LISTING APPENDIX SUBMITTED ON A COMPACT DISK[0003]NOT APPLICABLEBACKGROUND OF THE INVENTION[0004]The present invention provides compounds, pharmaceutical compositions containing one or more of those compounds or their pharmaceutically acceptable salts, which are effective in inhibiting the binding of various chemokines, such as MIP-1α, leukotactin, MPIF-1 and RANTES, to the CCR1 receptor. As antagonists or ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/53A61K31/506A61K31/501A61K31/496A61K31/495C07D403/02A61P29/00A61P37/00C07D231/12C07D231/14C07D231/16C07D231/18C07D231/38C07D231/56C07D233/54C07D235/06C07D249/12C07D257/04C07D401/04C07D401/12C07D403/04C07D403/12C07D405/04C07D409/04C07D473/34
CPCC07D231/12C07D473/34C07D231/16C07D231/18C07D231/38C07D231/56C07D233/56C07D235/06C07D249/12C07D257/04C07D401/04C07D401/12C07D403/04C07D403/12C07D405/04C07D409/04C07D231/14A61P1/00A61P17/00A61P25/00A61P25/04A61P29/00A61P37/00A61P37/08A61P43/00A61P9/00C07D401/06A61K31/496C07D401/14
Inventor PENNELL, ANDREW M.K.AGGEN, JAMES B.WRIGHT, J.J. KIMSEN, SUBRABRATAMCMASTER, BRIAN E.DAIRAGHI, DANIEL JOSEPH
Owner CHEMOCENTRYX INC
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