Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production of tyrosine hydroxylase positive neurons

a technology of tyrosine hydroxylase and positive neurons, which is applied in the field of neurons production, can solve the problems of inability to generate the required quantity of th expressing neurons, patients often become refractory to the continued effect,

Inactive Publication Date: 2005-07-07
STEM CELL THERAPEUTICS
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with chronic use of pharmacotherapy the patients often become refractory to the continued effect of L-DOPA (Marsden et al., 1977).
Since neurons are not capable of replicating, it is not practical to generate the required quantity of TH expressing neurons by culturing neurons in vitro.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

Induction of TH Expressing Neurons from Embryonic Neural Stem Cells

[0090] Neural stem cells were prepared from E14 medial and lateral ganglionic eminences and cultured in a culture medium (MHM) containing 20 ng / ml EGF to form neurospheres. The composition of MHM is as follows: [0091] DMEM / F12 (1:1) [0092] glucose (0.6%) [0093] glutamine (2 mM) [0094] sodium bicarbonate (3 mM) [0095] HEPES (5 mM) [0096] insulin (25 μg / ml) [0097] transferrin (100 μg / ml) [0098] progesterone (20 nM) [0099] putrescine (60 μM) [0100] selenium chloride (30 nM)

[0101] Seven days later, the neurospheres were passaged by mechanical dissociation and reseeded as single cells (passage 1). After cultured for seven days, the passage 1 neurospheres were completely dissociated and plated on poly-L-ornithine coated glass coverslips in the following medium: [0102] MHM [0103] BDNF (50 ng / ml) [0104] BMP2 (50 ng / ml)

[0105] 24 hours later, a TH cocktail was added to the media to the following final concentrations: [0106]...

example 2

Induction of TH Expressing Neurons from Adult Neural Stem Cells

[0113] Neural stem cells from adult brain were prepared from the subventricular zone of adult female CD 1 mice as described in Example 1. Thereafter, these stem cells were cultured to form neurospheres, passaged, plated on poly-L-ornithine coated glass coverslips, and incubated in TH medium as described in Example 1. After a two day incubation in the TH medium, 28% of β-tubulin positive cells expressed TH. Therefore, the neural stem cells derived from adult brain were also capable of differentiating into TH positive neurons in response to the TH cocktail.

example 3

The Effect of PACAP

[0114] Another protein kinase A activator, pituitary adenylate cyclase activating polypeptide (PACAP) was also tested in lieu of forskolin in the TH cocktail. Thus, a culture of neural stem cells was prepared and exposed to an TH cocktail as described in Example 1, except that 2 nM PACAP (American Peptide Company, Sunnyvale, Calif.) was used in the place of forskolin. The TH cocktail was replenished by 50% everyday for 4 days, and the cells were stained and counted as described in Example 1. A control experiments using 1 μM forskolin and no PACAP was performed in parallel as a comparison.

[0115] The results are summarized below:

TABLE 1The Effects of Forskolin and PACAPTH Cocktail 1TH Cocktail 2(Forskolin)(PACAP)Neurons (% of total cells)19% 23%TH (% of total cells) 2%6.4%TH (% of neurons)10.5%  27.8% 

[0116] Therefore, PACAP is effective in the production of TH positive neurons in the present invention. In fact, for the concentrations shown above, PACAP is more ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
concentrationsaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a method of producing neurons that express the enzyme tyrosine hydroxylase (TH) by subjecting neural stem cells to FGF-1, a protein kinase A activator, a protein kinase C activator, and dopamine / L-DOPA. Surprisingly, when forskolin is used as a protein kinase A activator, it requires only low levels of FGF-1 and forskolin to efficiently produce TH positive neurons from fetal or adult neural stem cells. Also provided are compositions used to produce TH positive neurons and the resulting neural cell culture, as well as a method of treating disease or conditions which are associated with dopamine neuron loss or dysfunction.

Description

RELATED APPLICATION [0001] This application claims the benefit of U.S. Provisional Application Ser. No. 60 / 282,918, filed Apr. 11, 2001, which is hereby incorporated by reference in its entirety.FIELD OF THE INVENTION [0002] The present invention relates to methods of producing neurons which express the enzyme tyrosine hydroxylase, including dopamine neurons. REFERENCES [0003] Du et al., “Multiple signaling pathways direct the initiation of tyrosine hydroxylase gene expression in cultured brain neurons”, Molecular Brain Research 50: 1-8 (1997a). [0004] Du et al., “Protein Kinase C Activators Work in Synergy with Specific Growth Factors to Initiate Hydroxylase Expression in Striatal Neurons in Culture”, J. Neurochemistry, 68: 654-569 (1997b). [0005] Du et al., “Synergy between Growth Factors and Transmitters Required for Catecholamine Differentiation in Brain Neurons”, J. Neuroscience, 15: 5420-5427 (1995). [0006] Du et al., “Novel expression of the tyrosine hydroxylase gene requires...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/18C12N5/0793
CPCA61K38/1825C12N5/0619C12N2501/01C12N2501/113C12N2501/35C12N2501/815C12N2501/70A61K2300/00A61P25/00
Inventor WEISS, SAMUELSHINGO, TETSURO
Owner STEM CELL THERAPEUTICS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com