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Rapid purity identification of rape seed

A seed and purity technology, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of time-consuming, labor-intensive, and expensive, and achieve the effect of rapid identification

Inactive Publication Date: 2005-07-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for the identification of the purity of rapeseed hybrid seeds, although there are three methods of grain morphology identification, seedling identification and field plot planting identification, there are many problems such as time-consuming, labor-intensive, and expensive in the actual application in hybrid rapeseed production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take for example the rapid identification of the purity of rapeseed male sterile line hybrids with light yellow leaf color marks or the purity of male sterile line breeding seeds. Sampling the hybrid or propagated CMS seeds with light yellow marks prepared by rapeseed male sterile lines, germinated indoors at room temperature (about 25° C.) for 1-2 weeks or sowed outdoors for 1-2 weeks. For hybrids, if the seedlings are normal green, it is a real hybrid, and if the leaves of the seedlings are light yellow, it is a sterile line, so as to intuitively and quickly count the proportion of mixed light yellow seedlings in the green seedlings, and determine the seed purity of hybrids ; For the sterile line, if the seedlings are normal green, it is a pollinated hybrid plant, and if the seedlings are yellow leaves, it is a sterile line, so as to intuitively and quickly count the proportion of mixed green seedlings in the yellow seedlings, and determine the sterile Seed purity of ...

Embodiment 2

[0015] Take the rapid identification of the purity of rapeseed male sterile line hybrids with yellow leaf color marks or the purity of male sterile line breeding seeds as an example. Sampling the hybrids or the propagated CMS seeds with yellow marks prepared by rapeseed male sterile lines, germinated indoors at normal temperature (about 25°C) for 1-2 weeks or sowed outdoors for 1-2 weeks. For hybrids, if the seedlings are normal green, it is a true hybrid, and if the seedlings have yellow leaves, it is a sterile line, so as to intuitively and quickly count the proportion of mixed yellow seedlings in green seedlings, and determine the seed purity of hybrids; For the CMS line, if the seedlings are normal green, it is a hybrid plant with pollen, and if the seedlings have yellow leaves, it is a CMS line. In this way, the proportion of mixed green seedlings in the yellow seedlings can be counted intuitively and quickly to determine the quality of the CMS line. Seed purity.

Embodiment 3

[0017] Take the rapid identification of the purity of rapeseed male sterile line hybrids with yellow-green leaf color marks or the purity of male sterile line breeding seeds as an example. Sampling the hybrid or propagated CMS seeds with the yellow-green mark rape CMS, germinated indoors at room temperature (about 25° C.) for 1-2 weeks or sowed outdoors for 1-2 weeks. For hybrids, if the seedlings are normal green, it is a true hybrid, and if the leaves of the seedlings are yellow-green, it is a sterile line. In this way, the proportion of mixed yellow-green seedlings in green seedlings can be counted intuitively and quickly, and the seed of hybrids can be determined. Purity; for the sterile line, if the seedlings are normal green, it is a hybrid plant, and if the seedlings are yellow-green leaves, it is a sterile line, so as to intuitively and quickly count the proportion of mixed green seedlings in the yellow-green seedlings, and determine whether the Seed purity of breeding...

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Abstract

A method for quickly discriminating the purity of rape seed includes such steps as choosing the hybridized seeds prepared from the sterile line of rape with leaf color marker or reproductive sterile-line seeds, indoor germinating test or outdoor sowing test, observing the ratio of the green seedlings to the seedlings with leaf color marker, and calculating to determine the purity of said seeds. Its advantages are high speed, and low cost.

Description

technical field [0001] The invention relates to a method for rapidly identifying the purity of rape seeds. Background technique [0002] Seed purity is the basis for high crop yield and the decisive factor for production promotion. Therefore, purity is one of the most important indicators of seed quality. In GB / T3543.1-7-1995 "Crop Seed Inspection Regulations", the three indicators of rapeseed, such as germination rate, clarity, and water content, can be inspected indoors, which is faster and takes a shorter time. Regarding the identification of the purity of rapeseed hybrid seeds, although there are three methods: grain morphology identification, seedling identification, and field plot planting identification, there are many problems such as time-consuming, labor-intensive, and costly in practical application in hybrid rapeseed production. Contents of the invention [0003] The purpose of the present invention is to provide an intuitive, economical and simple method for...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 吴殿星
Owner ZHEJIANG UNIV
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