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ELISA reagent box for determining antibody and antigen of swine circular virus II

A porcine circovirus and antigen detection technology, which is applied to the field of rapid diagnostic equipment for livestock diseases, can solve the problems of complicated test operation and long time-consuming, and achieves the effects of broad market prospects, simple and fast operation, and strong specificity.

Inactive Publication Date: 2005-02-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for sensitive testing of small amounts of DNA called PVC (polyvinyl chloride), used widely throughout medicine worldwide. It uses specialized molecules made from synthetic polymers like polyethyleneglycol (PEG). These materials have strong binding properties but they don't bind any other substances commonly found around us. They also work well even if there aren’t anything else we may want them added during production. Overall, this method simplifies the process of identifying pigments associated with diseases while reducing costs compared to traditional methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to the difficulty or inconvenience faced when testing PCV-2 because it involves complicated procedures involving multiple steps such as viral separation from samples collected during collection process, culturing them, measuring their ability to induce signs of illnesses like PSVs, IFI, IFNARPs, ELFIs, IPLS, LELSA, PRSS, rashes, lymphocyte fever, chickens kidneydiasis, hemorrhagie syndromes, acute respiratosis, pseudoacervulodesmata neonatal sepsis, pseudocystismatic joint disorders, bacterial meningoenesis, cyanosis, tissue degenerative processes, tumours, autoimmune phenomena, and vascular complications including pulmonarumisis and giant weight loss syndrome.

Method used

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  • ELISA reagent box for determining antibody and antigen of swine circular virus II
  • ELISA reagent box for determining antibody and antigen of swine circular virus II
  • ELISA reagent box for determining antibody and antigen of swine circular virus II

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] (3) Preparation of PCV2 antigen or antibody detection plate

[0025] Dilute anti-PCV2 dCap monoclonal antibody mAb1 with 0.01M carbonate buffer (pH9.6) to a final concentration of 50ng / ml, coat a detachable 96-well microtiter plate at 4°C overnight, and block with 0.1% BSA at 37°C for 1 hour , fully washed with PBS (pH7.4) washing solution (PBST) containing 0.05% Tween-20, and prepared an antigen detection plate; similarly, 1 μg / ml recombinant dCap protein was added to the antigen detection plate, and reacted at 37°C for 1 Hours, the PBST washing solution is fully washed, and then the antibody detection plate detection plate is prepared. Treat the antigen or antibody detection plate with 10% sucrose solution respectively, and use for PCV2 antigen or antibody detection ELISA kit assembly.

[0026] (4) Preparation of goat anti-pig IgG polyclonal antibody

[0027] Take pig whole blood, separate serum, prepare pig IgG protein with saturated ammonium sulfate, take 1 part o...

Embodiment 1

[0042] Example 1 PCV2 antigen detection ELISA kit

[0043] see figure 1 , prepare recombinant PCV2 dCap protein according to (1) method step in the embodiment, prepare anti-PCV2 dCap monoclonal antibody mAb1 and mAb2 according to (2) method step in the embodiment, prepare PCV2 antigen detection plate by (3) method step in the embodiment , prepare PCV2 antigen detection ELISA kit enzyme conjugate working solution according to (5) method step in the embodiment, prepare antigen detection ELISA kit positive control and negative control according to (6) method step in the embodiment, according to the embodiment (7) ) method steps to prepare sample diluent, 10 × concentrated washing solution, prepare chromogenic solution A, chromogenic solution B and stop solution according to (8) method steps in the embodiment, and finally, assemble various components into PCV2 antigen detection ELISA Reagent test kit.

[0044] When using the PCV2 antigen detection ELISA kit to detect porcine PCV...

Embodiment 2

[0045]Example 2 PCV2 antibody detection ELISA kit

[0046] see figure 2 , Preparation of recombinant PCV2 dCap protein, anti-PCV2 dCap monoclonal antibody mAb1, sample diluent, 10× concentrated washing solution, chromogenic solution A, chromogenic solution B, and the method steps of the stop solution are the same as those in the examples, and will not be repeated. . Prepare PCV2 antibody detection plate according to (3) method step in the embodiment, prepare PCV2 antibody detection ELISA kit enzyme conjugate working solution according to (4) or (5) method step in the embodiment, according to (6) method step in the embodiment Prepare the positive control and negative control of the antibody detection ELISA kit, and assemble the PCV2 antibody detection ELISA kit.

[0047] When using the PCV2 antibody detection ELISA kit to detect the level of pig PCV2 antibody, collect the blood of the pig to be tested, separate the serum, and dilute it with the sample diluent at a ratio of 1...

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Abstract

A PCV2 antigen or antibody kit for detecting ELISA is composed of antigen detection plate, enzyme conjunctive matter working solution, positive control, negative control, sample diluent, 10 x concentrated cleaning solution, colour development solution A and B as well as stop buffer.

Description

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Claims

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Application Information

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Owner ZHEJIANG UNIV
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