Fluorescent analysis reagent for testing blood homo cysteine, method for preparing same and use thereof

Abstract

A fluorescence analysis reagent kit for detecting homocysteine in blood is disclosed. It uses a novel internal standard thiohydroxy acetic acid for quantity control and the chromatographic peaks of said internal standard and homocysteine can be clearly separated from each other. Its advantages are less specimen, high correctness, high speed, and low cost.

Description

technical field [0001] The invention belongs to biotechnology diagnostic reagents, in particular to a fluorescent analysis reagent for detecting blood homocysteine ​​and its preparation method and application. Background technique [0002] In recent years, a large number of epidemiological and clinical case studies have proved that elevated blood homocysteine ​​is a more direct cause of arteriosclerosis and cardiovascular disease than cholesterol. Normal human blood homocysteine ​​averages 7-10 micromoles / liter, and more than 10 micromoles / liter is called homocysteinemia. At the end of 1999, the American Heart Association has called on physicians across the United States to test blood homocysteine ​​for patients with cardiovascular disease families and carry out clinical treatment to reduce blood homocysteine. The U.S. Drug and Food Administration (FDA) has ordered all manufacturers of finished cereals such as oatmeal to add folic acid and other ingredients t...

AI Technical Summary

Problems solved by technology

Although the British DREW company has developed a kit for the homocysteine ​​analyzer produced by the company, it cannot be used for other HPLC analyzers

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