Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Baffle slot type multilayer organ chip and method

A technology of organ chips and slots, which is applied in the fields of biomedical engineering and microfluidics, can solve the problems of hydrogels blocking the channels of culture fluid, achieve extended culture and co-culture of various cells, and improve cell culture efficiency Effect

Pending Publication Date: 2022-07-29
SHANGHAI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the current organ chip is injected with cell-containing hydrogel to block the culture medium channel, the present invention provides a multi-layer organ chip with baffle slots and a corresponding method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Baffle slot type multilayer organ chip and method
  • Baffle slot type multilayer organ chip and method
  • Baffle slot type multilayer organ chip and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A baffle slot type multi-layer organ chip, the structure of which is as follows Figure 1-Figure 8 As shown, it includes a liquid storage layer 1, a slot layer 2, a flow channel layer 3 and a bottom plate 4 that are stacked in sequence from top to bottom; the liquid storage layer 1 is provided with a first opening 11, and two sides of the first opening 11 are respectively provided with There are a left perfusion port group and a right perfusion port group which are gap-fitted with the first opening 11; the slot layer 2 is provided with a chip slot group, and the two sides of the chip slot group are respectively provided with a left channel which is gap-fitted with the chip slot group. The hole group and the right through hole group, the left through hole group and the right through hole group are matched with the left perfusion port group and the right perfusion port group respectively.

[0038] The flow channel layer 3 is provided with a second opening 31, and two side...

Embodiment 2

[0048] A method for assembling and culturing a multi-layer organ chip with a baffle slot type, the steps comprising:

[0049] Step 1. After assembling the liquid storage layer 1, the slot layer 2, the flow channel layer 3, and the bottom plate 4 of the multilayer organ chip in a certain order, seal the connection edges of each layer;

[0050] Step 2: Insert each chip baffle 5 into the first slot area 3121 , the third slot area 3123 and the fourth slot area 3124 in the chip slot area 312 through the first opening 11 and the chip slot 21 in sequence. ;

[0051] Step 3: Perfuse the hydrogel containing cell A into the second chamber 3112 of the inner chamber 311 of the second opening 31 through the corresponding left perfusion port 12 , the left through hole 22 , the left flow channel port 32 , and the left flow channel 34 respectively. glue, pour the hydrogel containing cell A into the third chamber 3113, wait for the hydrogel to solidify, remove the first slot area 3121 in the ...

Embodiment 3

[0054] A method for assembling and culturing a multi-layer organ chip with a baffle slot type, the steps comprising:

[0055] Step 1. After assembling the liquid storage layer 1, the slot layer 2, the flow channel layer 3, and the bottom plate 4 of the multilayer organ chip in a certain order, seal the connection edges of each layer;

[0056] Step 2: Insert each chip baffle 5 into the first slot area 3121 , the second slot area 3122 , the third slot area 3123 and the in the fourth slot area 3124;

[0057] Step 3: Perfuse the hydrogel containing cells A into the second chamber 3112 in the inner chamber 311 of the second opening 31 through the corresponding left perfusion port 12 , the left through hole 22 , the left flow channel port 32 , and the left flow channel 34 respectively. , pour the hydrogel containing cell B into the third chamber 3113, wait for the hydrogel to solidify, remove the first slot area 3121, the second slot area 3122 and the third slot area 3122 in the ch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
widthaaaaaaaaaa
Login to View More

Abstract

The invention provides a baffle slot type multi-layer organ chip. The baffle slot type multi-layer organ chip comprises a liquid storage layer, a slot layer, a runner layer and a bottom plate which are sequentially stacked from top to bottom, a first opening is formed in the liquid storage layer, and a left filling port group and a right filling port group which are in clearance fit with the first opening are respectively formed in two sides of the first opening; the slot layer is provided with a chip slot group, two sides of the chip slot group are respectively provided with a left through hole group and a right through hole group which are in clearance fit with the chip slot group, and the left through hole group and the right through hole group are respectively matched with the left filling port group and the right filling port group; the liquid storage layer, the slot layer, the flow channel layer and the bottom plate which are arranged in a stacked mode form the multi-layer trachea chip, hydrogel containing cells is poured into the cavity through the left pouring opening, the left through hole, the left flow channel opening and the left flow channel in sequence, the chip baffle serves as a temporary pouring barrier, and the hydrogel containing the cells is blocked in the designated cavity.

Description

technical field [0001] The invention belongs to the technical fields of biomedical engineering and microfluidics, in particular to a baffle slot-type multilayer organ chip, and also relates to a method for the baffle slot-type multilayer organ chip. Background technique [0002] Organ-on-a-chip is a microfluidic cell culture system fabricated by a microfabrication process, which can culture cells in three dimensions outside the human body to simulate organ functions in the human body. Organ-on-a-chip has good application prospects in the fields of drug development and screening, disease model evaluation, and personalized medicine. [0003] Toxicological testing and safety verification of drugs must be carried out before the drug is put on the market. Traditional drug toxicological testing is usually carried out in two-dimensional cell models or animals. Two-dimensional cell culture models are difficult to simulate complex physiological activities in human tissues and organs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/00B01L3/00
CPCC12M23/16C12M27/20C12M29/10C12N5/0062C12N5/0068B01L3/502723C12N2513/00C12N2535/00
Inventor 岳涛姜宁蔚延聪张鑫业张泉李龙钟宋义李恒宇谢少荣罗均
Owner SHANGHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com