Feed additive for relieving hepatic glycogen accumulation and application thereof

A feed additive and hepatic glycogen technology is applied in the field of feed additives for relieving hepatic glycogen accumulation, which can solve the problems of ineffective hepatic glycogen accumulation syndrome, avoid sugar from entering the blood or accumulate in the liver, and achieve precise and efficient regulation. high effect

Pending Publication Date: 2022-06-17
WUHAN SUNHY BIOLOGICAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the problem that the effec

Method used

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  • Feed additive for relieving hepatic glycogen accumulation and application thereof
  • Feed additive for relieving hepatic glycogen accumulation and application thereof
  • Feed additive for relieving hepatic glycogen accumulation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025]实施例1：

[0026]本实施例提供了一种用于缓解肝糖原累积的饲料添加剂，包括按重量份数计的如下组分：10葡萄糖氧化酶、10份枯草芽孢杆菌、5份单宁酸和75份生物载体。将上述各原料按比例称量好后置于混合机中混合30分钟后得到饲料添加剂。

[0027]其中，每克复合酶制剂中葡萄糖变旋酶的酶活为500U，葡萄糖氧化酶的酶活为2000U，过氧化氢酶的酶活为300U；枯草芽孢杆菌为武汉新华扬公司提供的由ACCC11088菌株生产的具有淀粉酶活性的枯草芽孢杆菌；单宁酸为五倍子水解产生的单宁酸；生物载体为稻壳粉，其水分含量为8％，且90％稻壳粉可以通过80目的饲料筛。

[0028]配制低糖饲料，包括：5％的国产鱼粉、43.85％的进口鱼粉、9％的宠物级鸡肉粉、5％的血浆蛋白粉、3％的谷朊粉、6％豆粕、2.5％的花生粕、2％的米糠、4％的面粉、4％的木薯粉、1％的酵母、5％的膨润土、1.5％的磷酸二氢钙、3％的鱼油、3％的豆油、1％的维生素预混料、1％的矿物质预混料和0.15％的氯化胆碱。

[0029]配制高糖饲料，包括：5％的国产鱼粉、43.85％的进口鱼粉、9％的宠物级鸡肉粉、5％的血浆蛋白粉、3％的谷朊粉、6％豆粕、2％的花生粕、1％的米糠、7％的面粉、7％的木薯粉、1％的酵母、0.5％的膨润土、1.5％的磷酸二氢钙、3％的鱼油、3％的豆油、1％的维生素预混料、1％的矿物质预混料和0.15％的氯化胆碱。

[0030]将300尾大口黑鲈随机分为实验组、对照组1、对照组2和对照组3，每组三个重复，每个重复25尾大口黑鲈。

[0031]实验组：饲喂高糖饲料+0.1％的上述用于缓解肝糖原累积的饲料添加剂；

[0032]对照组1：饲喂低糖饲料；

[0033]对照组2：饲喂高糖饲料；

[0034]对照组3：饲喂高糖饲料+0.1％的市售添加剂。

[0035]每天8:30、16:30各投喂1次，表观饱食投喂，养殖时间8周。

[0036]养殖试验结束后，所有试验鱼称重，统计每个重复饲料投喂量，用以计算生长性能和饲料利用，结果如表1所示。

[0037]表1生长性能和饲料利用率

[0038]

[0039]从表1可以看出，与对照组2和对照组3相比，在大口黑鲈高糖饲料中添加本发明提供的饲料添加剂后，显著提高试验鱼的末体重、增重率的同时，也降低了饲料系数、饲料成本和死亡率，有利于提高养殖效益和成活率。

[0040...

Example Embodiment

[0045]实施例2：

[0046]本实施例提供了一种用于缓解肝糖原累积的饲料添加剂，包括按重量份数计的如下组分：20葡萄糖氧化酶、30份枯草芽孢杆菌、10份单宁酸和40份生物载体。将上述各原料按比例称量好后置于混合机中混合30分钟后得到饲料添加剂。其中，每克复合酶制剂中葡萄糖变旋酶的酶活为1000U，葡萄糖氧化酶的酶活为4000U，过氧化氢酶的酶活为500U；枯草芽孢杆菌为武汉新华扬公司提供的由ACCC11088菌株生产的具有淀粉酶活性的枯草芽孢杆菌；单宁酸为五倍子水解产生的单宁酸；生物载体为玉米芯粉，其水分含量为8％，且90％玉米芯粉可以通过80目的饲料筛。

[0047]以0.05％的添加量将上述用于缓解肝糖原累积的饲料添加剂加入市售加州鲈饲料中，喷入淀粉浆，使饲料添加剂粘附与饲料上。

[0048]选择大小一致，鱼苗放养量相当的两口大口黑鲈养殖池塘进行实验，其中1号塘作为对照组，投喂市售加州鲈饲料，2号塘作为实验组，投喂上述添加有用于缓解肝糖原累积的饲料添加剂的市售加州鲈饲料。

[0049]投喂一周后，用撒网从每口池塘中随机捕出10尾大口黑鲈，解剖观察其肝脏颜色，投喂了本发明添加剂的大口黑鲈肝脏较未投喂本发明添加剂的大口黑鲈的肝脏更加红润。实验组和对照组各选取两尾鱼，将肝脏制作切片进行比较，如图2所示，对照组的1号样本中肝细胞中大部分细胞核消失，出现较大面积的空泡变性，图3中对照组的2号样本管中的肝脏有轻微的空泡变性。而如图4-5所示，实验组对应的3号样本和4号样本中的肝细胞中大部分细胞核正常，核质充盈，极少数肝细胞出现空泡变性。

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Abstract

The invention belongs to the technical field of breeding feed, and particularly provides a feed additive for relieving hepatic glycogen accumulation, which comprises the following components in parts by weight: 10-20 parts of compound glucose oxidase, 10-30 parts of bacillus subtilis, 5-10 parts of tannic acid and 40-75 parts of biological carrier. When being used in the largemouth bass feed, the bacillus subtilis can reduce the accumulation of hepatic glycogen through three checks, regulate and control glycometabolism from the source, and reduce the conversion of starch in the feed to sugar and the absorption of the organism to the sugar, thereby preventing excessive sugar from entering blood or being accumulated in the liver, and improving the immunity of the largemouth bass. The problems of frequent hepatic glycogen accumulation syndrome and high death rate of the micropterus salmoides fed with artificial compound feed are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of breeding feed, and in particular relates to a feed additive for alleviating the accumulation of liver glycogen and its application. Background technique [0002] Largemouth bass, commonly known as California sea bass, has become one of the main breeds in my country due to its delicious meat and high nutritional value, and has the reputation of "the fifth most popular fish". In recent years, with the expansion of breeding scale and the improvement of environmental protection requirements, the breeding of largemouth bass has gradually changed from feeding iced fish to using compound feed. Hepatic glycogen accumulation syndrome, which affects the promotion of largemouth bass compound feed and the further development of largemouth bass aquaculture to a certain extent, therefore, it has become an effective way to alleviate the liver glycogen accumulation syndrome caused by feeding compound feed The key point ...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/163A23K20/189A23K10/30A23K10/37A23K50/80
CPCA23K10/18A23K20/163A23K20/189A23K10/30A23K10/37A23K50/80Y02P60/87
Inventor 吴建军朱传忠张成杰周樱宋雪荣辜玲芳
Owner WUHAN SUNHY BIOLOGICAL
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