Molecular marker for identifying fruit mulberry variety 'Guangdong mulberry 143', identification primer group, kit and application

Abstract

The invention discloses a molecular marker for identifying a fruit mulberry variety 'Guangdong mulberry 143', an identification primer group, a kit and application. The fluorescent SSR technology is utilized, 300 pairs of SSR primers designed and developed for the mulberry genome sequence are screened, it is confirmed that the core primers marked with M20541 and M22794 can amplify three specific bands for the new fruit mulberry variety 'Yuegui 143' respectively, the new fruit mulberry variety 'Yuegui 143' can be identified from the fruit mulberry strains respectively, and the specific bands of the new fruit mulberry variety 'Yuegui 143' and the specific bands of the new fruit mulberry variety 'Yuegui 143' can be identified from the fruit mulberry strains respectively. The method can be used for rapid identification and detection of a new fruit mulberry variety 'Guangdong mulberry 143'. According to the method, the M20541 marker and / or the M22794 marker of 'Yueyan mulberry 143' are / is directly detected to identify the 'Yueyan mulberry 143', the uncertainty of external morphological characteristics on identification of the 'Yueyan mulberry 143' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry 'Yueyan mulberry 143', and the method is beneficial to popularization, utilization and protection of the 'Yueyan mulberry 143'.

Description

technical field [0001] The invention relates to the technical field of identification of variety resources and germplasm innovation, in particular to a molecular marker for identification of the fruit mulberry variety 'Yuechen 143', identification primer set, kit and application. Background technique [0002] Mulberry fruit is a kind of characteristic and excellent fruit resource, and it is the representative of the third-generation fruit. It has been listed as "both food and medicine" by the Ministry of Health. In recent years, with the improvement of people's living standards and health awareness The nutritional and health value of mulberry fruit is increasingly concerned by the society. As a new fruit variety, mulberry fruit is increasingly loved by people because of its unique taste, rich nutrition and high active substances. Into the families of ordinary people, accepted and loved by the citizens. In addition to fresh food, mulberries are processed and developed into m...

AI Technical Summary

Problems solved by technology

[0005] The traditional identification of new mulberry varieties is mainly based on phenotypic traits, which is greatly affected by the environment, poor stability, and long testing cycle, which seriously affects the effectiveness and authority of new variety identification

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