Organoid culture device and organoid culture method

Abstract

The invention belongs to the technical field of cell culture, and particularly discloses an organoid culture device and an organoid culture method. The organoid culture device comprises a supporting piece and a separating piece, wherein the supporting piece is provided with a culture tank; the separating piece is positioned in the culture tank; a liquid flowing gap is formed between the lower surface of the separating piece and the tank bottom of the culture tank; and the separating piece is provided with liquid changing through holes and a plurality of culture through holes which respectively communicate with the liquid flowing gap. The culture through holes separate a plurality of organoid cell balls, so that the phenomenon that two or more organoid cell balls adhere to each other is avoided, contact exchange between nutrient substances and oxygen and the center part of the organoid is facilitated, and the culture effect of the organoid cell balls is ensured; and moreover, no organoid cell ball is arranged in the liquid changing through holes, so that when cell liquid changing treatment is performed, the organoid cell balls with smaller volume can be prevented from being sucked away, the organoid cell balls with larger volume can be prevented from being damaged, and the normal growth of the organoid cell balls is ensured.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an organoid culture device and an organoid culture method. Background technique [0002] At present, organoid culture techniques are mainly divided into three-dimensional culture techniques that rely on exogenous scaffolds and three-dimensional culture techniques that do not rely on exogenous scaffolds. The three-dimensional culture technology that does not rely on exogenous scaffolds mainly uses physical methods to aggregate cells suspended in the culture medium to a certain density to form tight cell-cell connections, so that they can self-assemble into three-dimensional organoid structures that do not rely on exogenous scaffold structures. . The technical methods currently applied to the market mainly include suspension culture method, hanging drop culture method and low adhesion surface method. Among them, the suspension culture method can prepare a large number of org...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide an organoid culture device to solve the problems in the prior art that multiple organoid cell spheroids are easy to stick together, smaller organoid cell spheroids are easily sucked away when the liquid is changed, and larger organoid cell spheroids are easily absorbed. The technical problem that organ cell spheroids are easily destroyed

Images