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SNP (Single Nucleotide Polymorphism) molecular marker related to muscle drip loss character of meat rabbit and application of SNP molecular marker

A technology of molecular markers and drip loss, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as unsound disease prevention and control system, degradation of meat rabbit breeds, and influence on the development of rabbit industry

Active Publication Date: 2021-11-30
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the breed of meat rabbits is seriously degraded, the feed nutrition is unbalanced, the disease prevention and control system is not perfect, and the breeding technology is backward, which affects the development process of the entire rabbit industry.

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker related to muscle drip loss character of meat rabbit and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related to muscle drip loss character of meat rabbit and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related to muscle drip loss character of meat rabbit and application of SNP molecular marker

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Embodiment 1

[0024] 1. Source of test animals

[0025] The experimental meat rabbit breed used in this embodiment is as follows:

[0026] Fujian Yellow Rabbit, provided by the Natural Ecological Agriculture Experimental Field in Lianjiang Yuhuashan, Fujian Province;

[0027] Southwest Fujian Black Rabbit, provided by Longyan Tongxian Rabbit Industry Development Co., Ltd.;

[0028] New Zealand white rabbits and Fujian white rabbits were provided by the Fujian White Rabbit Breeding Farm in Yuantian Village, Wudong Township, Wuping County.

[0029] 2. Drip Loss Determination of Rabbit Meat

[0030] Within 1-2 hours after the meat rabbit was slaughtered, take the longissimus dorsi muscle with a length of about 2.5cm at the 4-7 lumbar vertebrae and weigh it, hang it in a refrigerator at 4°C for storage, take it out after 24 hours, and dry the meat sample with filter paper surface moisture and weighed again. Drip loss (%) = (weight of meat sample before storage - weight of meat sample after ...

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Abstract

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a muscle drip loss character of a meat rabbit and application of the SNP molecular marker. The SNP molecular marker is located in a 3' end non-coding region of a meat rabbit FABP4 gene, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1, a C / T mutation exists at the 101th basic group of the sequence, the mutation causes the nucleotide of the sequence to generate polymorphism, and when the genotype of the locus is TT, the meat rabbit to be detected belongs to an individual with low muscle drip loss.

Description

technical field [0001] The invention belongs to the technical field of preparation of rabbit molecular markers, and in particular relates to a SNP molecular marker related to muscle drip loss traits of meat rabbits and an application thereof. Background technique [0002] Meat rabbits have the advantages of high economic value, wide application, high yield, low consumption, and quick results. With the continuous improvement of people's living standards and foreign exports, rabbit breeding has become a new bright spot in animal husbandry and has broad development prospects. However, the breed of meat rabbits has degenerated seriously, the feed nutrition is unbalanced, the disease prevention and control system is not perfect, and the breeding technology is backward, which affects the development process of the entire rabbit industry. The seed industry is one of the most important links affecting the rabbit industry, and the key to determining the production level of meat rabbi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172Y02P60/87
Inventor 桑雷王锦祥陈冬金孙世坤高晨芳谢喜平陈岩锋
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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