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Preparation method of high-activity lactobacillus fermentation extracting solution

A technology for the fermentation and extraction of lactic acid bacteria, which is applied in the field of preparation of lactic acid bacteria fermentation extracts, can solve the problems of lactic acid bacteria fermentation products such as high temperature resistance, failure to maintain lactic acid bacteria activity, and inability to maintain lactic acid bacteria activity, etc., to improve skin microecology and protect skin function, the effect of improving tolerance

Active Publication Date: 2021-09-17
绽妍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when adding substances to promote the proliferation of lactic acid bacteria or mixing substances to improve the viability of lactic acid bacteria to obtain sufficient effects, the more these substances are used, the more the quality of the product will be affected, and it will also cause the lactic acid bacteria fermented product Acid resistance, alkali resistance, high temperature resistance and other performance degradation
Therefore, even if it can maintain a state containing a large amount of live lactic acid bacteria, it cannot maintain the activity of lactic acid bacteria, let alone maintain the activity of lactic acid bacteria under extreme conditions

Method used

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  • Preparation method of high-activity lactobacillus fermentation extracting solution
  • Preparation method of high-activity lactobacillus fermentation extracting solution

Examples

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Effect test

Embodiment 1

[0027] A method for preparing a highly active lactic acid bacteria fermentation extract: comprising the following steps:

[0028] S1, select pandanus and mulberry fruit to wash, crush respectively, add 0.1% antioxidant of the total mass of pandanus and mulberry fruit to squeeze the juice, the antioxidant consists of thymol and carvacrol with a mass ratio of 0.1:2:1.3 and coumarin are mixed, filtered through 120 mesh, and the filter residue is taken to obtain pandanus pomace and mulberry pomace;

[0029] S2. Preparation of pandanus fruit extract: the pandanus pomace obtained in S1 was first extracted with 60wt% ethanol solution for 1 hour, and then extracted with 70wt% ethyl acetate solution for 0.5 hours for the second time, and combined The extract is concentrated under reduced pressure and dried after purification to obtain the pandanus extract; the mass volume ratio of pandanus pomace and ethanol solution g / mL is 1:20, and the mass volume of pandanus pomace and ethyl acetat...

Embodiment 2

[0035] A method for preparing a highly active lactic acid bacteria fermentation extract: comprising the following steps:

[0036] S1, select pandanus and mulberry fruit to wash, crush respectively, add 0.5% antioxidant of the total mass of pandanus and mulberry fruit to squeeze the juice, the antioxidant consists of thymol and carvacrol with a mass ratio of 0.5:5:2 and coumarin are mixed, filtered through 150 mesh, and the filter residue is taken to obtain pandanus pomace and mulberry pomace;

[0037] S2. Preparation of pandanus extract: the pandanus pomace obtained in S1 is first extracted with 80wt% ethanol solution for the first 3h, and then extracted with 90wt% ethyl acetate solution for the second time for 2h, combined for extraction liquid, concentrated under reduced pressure, and dried after purification to obtain pandanus fruit extract; the mass volume ratio g / mL of pandanus pomace and ethanol solution is 1:45, and the mass volume ratio of pandanus pomace and ethyl ace...

Embodiment 3

[0043] A method for preparing a highly active lactic acid bacteria fermentation extract: comprising the following steps:

[0044] S1, select pandanus and mulberry fruit to wash, crush respectively, add 0.3% antioxidant of the total mass of pandanus and mulberry fruit to squeeze the juice, the antioxidant consists of thymol and carvacrol with a mass ratio of 0.3:4:1.7 and coumarin are mixed, filtered through 130 mesh, and the filter residue is taken to obtain pandanus pomace and mulberry pomace;

[0045] S2. Preparation of pandanus fruit extract: the pandanus pomace obtained in S1 was first extracted with 70wt% ethanol solution for the first 2h, and then leached for the second time with 80wt% ethyl acetate solution for 1.6h, and combined The extract is concentrated under reduced pressure, dried after purification, and the pandanus extract is obtained; the mass volume ratio g / mL of pandanus pomace and ethanol solution is 1:35, and the mass volume of pandanus pomace and ethyl ace...

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Abstract

The invention provides a preparation method of a high-activity lactobacillus fermentation extracting solution, which comprises the following steps: S1, pretreating pandanus tectorius and mulberry fruits to obtain pandanus tectorius pomace and mulberry pomace; S2, preparing a pandanus tectorius extract through alcohol extraction and secondary extraction; S3, preparing a mulberry fruit extract, performing extracting with an ethanol solution, and preparing the mulberry fruit extract under an ultrasonic condition; S4, preparing a lactobacillus suspension; S5, fermenting: inoculating the lactobacillus suspension with the pandanus tectorius extract and the mulberry fruit extract, and adjusting the ratio and fermentation conditions to obtain a lactobacillus fermentation solution; and S6, performing extracting with ethanol to obtain the high-activity lactobacillus fermentation extracting solution. The lactobacillus fermentation extracting solution disclosed by the invention is relatively high in activity under high temperature, acid and alkali conditions, high in viable bacteria value, can be applied to the fields of daily necessities and cosmetics, can be used as an antioxidant and a skin conditioner, can improve the skin micro-ecology, and has the effects of resisting oxidation and resisting aging on the skin.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for preparing a highly active lactic acid bacteria fermentation extract. Background technique [0002] Probiotics such as Lactobacillus and Bifidobacterium have been widely used in the food industry. At present, it is believed that these bacteria not only do no harm to human health, but also have health care effects on the human body, can improve the balance of microorganisms in the host intestine, and promote intestinal Digest and absorb, improve immune function. [0003] Patent "Original lactic acid bacteria beverage with compound lactic acid bacteria fermentation flavor and its preparation method" publication number: CN107712047A, fermentation is carried out by adding compound stabilizer, compound sugar, etc., but the main active substance content of lactic acid bacteria fermentation is low; [0004] Patent "Lactic acid bacteria fermented product and fermented ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/99A61Q19/08
CPCA61K8/9789A61K8/99A61Q19/08A61K2800/522A61K2800/85A61K2800/5922A61K2800/82
Inventor 舒凌李嘉文
Owner 绽妍生物科技有限公司
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