A scalable and universal cancer organoid culture device in vitro

Abstract

The invention discloses a medical device, in particular to an expandable structure and universal cancer organoid in vitro culture device. A mounting part is slidably arranged on the guide rail. One side of the mounting part is rotated to install a residual gear. The rack plate matched with the residual gear is installed horizontally on the side wall of the No. 2 point plate; it also includes the power assembly installed on the mounting part, the sampling assembly that is movably arranged on the mounting part and connected to the power assembly, and the sample supply Components; when the residual gear rotates, the toothed part on it is intermittently combined with the rack plate on the second point plate, driving the second point plate to move intermittently horizontally, and the elastic structure drives the first point plate to rise to be flush with the second point plate , the mounting part moves intermittently horizontally with the residual gear under the action of the guide rail; the worm drives the worm gear and the sample spotting assembly to move synchronously, and the sample spotting assembly drips cancer cell matrix gel into the spot pits arranged in array on the spotting plate, and then pours cancer cell matrix gel onto the second spotting plate Add cancer cell Matrigel evenly to the No. 1 spot plate for culture.

Description

technical field [0001] The invention relates to a medical device, in particular to an expandable structure and universal cancer organoid in vitro culture device. Background technique [0002] In recent years, in vitro cell culture technology has achieved rapid development. Now cell culture is not just the traditional planar culture mode, and cells can also form three-dimensional structures in vitro through various methods. [0003] The current organ culture technology mainly uses stem cells. By simulating the environment of human development, stem cells are induced to differentiate into different types of cells in an organ and form the basic structure of the organ. The cancer tissue samples were cut and digested, and the cancer cells obtained after digestion were resuspended with pre-cooled Matrigel, the cancer cell suspension was spot-plated, and the spot-plated cancer cells were treated Culture treatment, and finally obtain cancer organoids. [0004] Spotting treatment i...

AI Technical Summary

Problems solved by technology

[0004] Spotting treatment is to drop the matrix gel of cancer cells onto the TLC plate in a matrix, and then culture them. Most of the existing spotting culture methods are manually operated. Since the success rate of culture is not 100%, multiple cultures need to be cultured. Larger TLC plates are required, and the stroke requirements of the equipment are higher if samples are spotted by the machine, so only a small amount of culture can be used, which is not conducive to simultaneous culture of multiple organs

Images