Detection substance for SNP of ONFH susceptibility related VDR, MMP2, MMP3 and MMP9 genes and application

A gene and substance technology, which is applied to the detection substances and application fields of ONFH susceptibility-related VDR, MMP2, MMP3, and MMP9 gene SNPs, can solve the problem of less data on multi-gene loci, more data on gene loci, and various disadvantages. Problems such as joint pathogenicity of minor genes

Pending Publication Date: 2021-02-26
JILIN UNIV
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Problems solved by technology

[0023] However, at present, there are major bottlenecks in the research field of ONFH gene polymorphism at home and abroad. There are many data on scattered research gene loci, and there is little data on systematic research on multiple gene loci, which is not conducive to the joint pathogenicity of multiple minor genes. Elaboration of molecular etiology and molecular pathogenesis

Method used

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  • Detection substance for SNP of ONFH susceptibility related VDR, MMP2, MMP3 and MMP9 genes and application
  • Detection substance for SNP of ONFH susceptibility related VDR, MMP2, MMP3 and MMP9 genes and application
  • Detection substance for SNP of ONFH susceptibility related VDR, MMP2, MMP3 and MMP9 genes and application

Examples

Experimental program
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Embodiment 1

[0087] Example 1. Application of VDR, MMP2, MMP3, and MMP9 gene polymorphism combinations in detecting molecular early warning, molecular diagnosis and therapeutic drug targets of non-traumatic femoral head necrosis.

[0088] 1. Establish ONFH case-control study system

[0089] Based on informed consent, the ONFH case-control study system included 600 cases, including 300 cases in the healthy control group and 300 cases in the ONFH patient group.

[0090] ONFH cases came from inpatient clinical patients who visited the Department of Orthopedics of the Second and Third Clinical Medical Colleges of Jilin University from June 2012 to October 2018, including 204 males and 96 females, aged 54.49±11.8 years. The clinical diagnosis and staging of ONFH were performed according to the Ficat diagnosis and staging criteria, excluding obvious hip trauma history, congenital hip joint diseases, hip joint infectious diseases and hip joint tumors.

[0091] The healthy control group included ...

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Abstract

The invention provides 11SNPs markers of MMP3, MMP9, MMP2 and VDR genes or a combination of the markers. The 11SNPs markers or a combination are used for ONFH molecular early warning, clinical molecular diagnosis and typing, and drug therapy targets. The pairwise interaction of the 11SNPs is significantly related to the onset risk of ONFH, and the risk correlation is partially or completely different from the action of a single SNP on the onset risk of the ONFH, the synergistic effect of a plurality of SNPs on the onset risk of the ONFH is found more clearly according to the result of the geneinteraction, the ONFH is further verified to be a complex disease caused by combination of multiple micro-effect genes, the joint effect of the molecular genetics sites is elaborated, and the important value is achieved for ONFH early-stage molecular early warning and molecular prevention and control strategy establishment.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to MMP3 gene single nucleotide polymorphic sites rs650108, rs679620, rs522616, MMP9 gene single nucleotide polymorphic sites rs17576, rs3918249, rs3918241, rs2274755, MMP2 gene single nucleotide polymorphic sites Application of state site rs243847, rs243832, rs14070 and VDR gene single nucleotide polymorphic site rs731236 marker or a combination of markers in the preparation of diagnosis or auxiliary diagnosis, preparation evaluation or auxiliary evaluation of products with ONFH risk of human beings to be tested , and further used for ONFH molecular early warning, clinical molecular diagnosis and typing, and drug therapy targets. Background technique [0002] Osteonecrosis of the femoral head (ONFH) is a complex disease caused by the joint action of genetic factors and environmental factors. In the past 10 years, the incidence of ONFH has shown an increasing trend year by year. It is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P19/08
CPCC12Q1/6883A61K45/00A61P19/08C12Q2600/156C12Q2600/112C12Q2600/106
Inventor 宋旸李忱陈高扬杜珍武张桂珍秦彦国王金成
Owner JILIN UNIV
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