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Visual detection method for tumor metastasis related neutrophils

A technology for neutrophils and tumor metastasis, which is applied in the field of visualization and detection of circulating tumor cells captured by tumor metastasis-related neutrophils, and can solve the problems of difficulty in extending to various experimental platforms, high technical requirements, and expensive visualization devices.

Active Publication Date: 2020-12-04
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing visualization devices for tumor metastasis are expensive and technically demanding, making it difficult to extend to various experimental platforms

Method used

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Embodiment Construction

[0014] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0015] The invention provides a method for visually detecting neutrophils related to tumor metastasis, which specifically includes the following steps:

[0016] 1) Prepare new tumor metastasis-associated neutrophils: use flow cytometry to obtain tumor metastasis-associated neutrophils from peripheral blood of breast cancer mice and patients, and evenly spread them on poly-lysine pre-coated 12-well plates Incubate for 2 hours, fix with paraformaldehyde, and use 1 μM orange nucleic acid dye (syto...

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Abstract

The invention discloses a visual detection method for tumor metastasis related neutrophils. The visual detection method specifically comprises the following steps of 1) preparing novel tumor metastasis related neutrophils, 2) preparing tumor cells, 3) preparing a visual device, 4) preparing a fluid, 5) preparing a recording device, 6) searching an optimal visual field, and 7) recording tumor metastasis related neutrophil capture circulating tumor cells. The in-vivo fluid condition of tumor cells captured by novel tumor metastasis related neutrophils can be simulated by utilizing existing equipment in a laboratory, visual detection is carried out, high-end precious equipment does not need to be specially purchased, and the method can be popularized and used in an experimental platform.

Description

technical field [0001] The invention relates to the experimental field of tumor metastasis detection, in particular to a visual detection method for tumor metastasis-related neutrophils capturing circulating tumor cells. Background technique [0002] At present, a group of novel tumor metastasis-associated neutrophils that promote breast cancer lung metastasis have been found in humans and mice, with unique functional phenotypes, expressed as CD45+CD11b+CD66b+CXCR4+CD62Llow (human), and CD45+ CD11b+Ly-6G+CXCR4+CD62Llow (mouse). Existing visualization devices for tumor metastasis are expensive and require high technology, making it difficult to be extended to various experimental platforms. Contents of the invention [0003] In view of the above-mentioned technical deficiencies, the purpose of the present invention is to provide a visual detection method for neutrophils associated with tumor metastasis, which utilizes the existing equipment in the laboratory to simulate th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 黄建王震杨承慧李丽丽潘鋆
Owner ZHEJIANG UNIV
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