A microbial detection system

Abstract

The invention relates to a preparation method of a vertical microbiological detection chip, through which a differentiated mask is used to form a penetrating and / or non-penetrating microchannel structure on each sheet; then the materials of each sheet are combined to obtain The water transport channel and the accommodation chamber for containing micro-droplets on a single sheet, or the combined transport channel and combined accommodation chamber formed by a combination of multiple sheets, the chip prepared by the present invention also has the ability to induce micro-fluid The droplet enters the side flow channel of the holding chamber, which can have the same depth as the holding chamber, or is only provided in the middle layer, thereby having a depth smaller than that of the holding chamber, by setting the microchannel structure of the middle layer at Compared with the microchannel structure of other sheets, it has stronger hydrophilic characteristics, allowing the use of a reduced cross-sectional area of ​​the lateral flow channel to achieve the same lateral flow intensity.

Description

technical field [0001] The invention relates to a biological detection device, in particular to a microbial detection system for performing specific DNA detection of microorganisms such as viruses and bacteria through PCR reactions. Background technique [0002] The digital PCR chip based on micro-droplets is one of the most advanced nucleic acid quantitative detection methods. Compared with traditional PCR technology, digital PCR dilutes the solution containing target genes, primers, polymerases, etc., and divides them into dozens to dozens Tens of thousands of tiny, independent reactors, so that the number of nucleic acid templates in each reactor is less than or equal to 1, and each reactor is subjected to traditional PCR amplification and fluorescence detection. The reactor containing the target gene is marked as 1, and the reactor without the target gene is marked as 0. According to the relative ratio and the volume of the reactor, the nucleic acid concentration of the ...

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Problems solved by technology

However, due to the resistance of the microchannel itself and the local barrier, there is a large pressure difference in the continuous phase fluid flowing between the upstream and downstream of the distribution channel, which will lead to a huge difference in the filling effect of the micro-reaction chamber at the upstream and downstream of the distribution channel.

[0005] In addition, in the process of fluorescence detection of the amplified micro-reaction chamber, the reflection and refraction of the incident excitation light often cause strong background interference; Due to the non-directional nature of the fluorescence emitted by the stimulated micro-droplets in the reaction chamber, some of the fluorescence signals between adjacent micro-wells often pass through the hole wall and are mixed with the fluorescent signals from adjacent droplets, making it difficult to distinguish The problem

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