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A method and application for constructing retinal neovascular disease model

A new blood vessel, disease model technology, applied in the biological field, can solve the problem of lack of animal models

Active Publication Date: 2021-02-02
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is still a lack of animal models available for RNV therapeutic research

Method used

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  • A method and application for constructing retinal neovascular disease model
  • A method and application for constructing retinal neovascular disease model
  • A method and application for constructing retinal neovascular disease model

Examples

Experimental program
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Embodiment 1

[0036] This embodiment provides a method for constructing a retinal neovascular disease model, which includes knocking out the second exon of the Ctnna1 gene in the vascular endothelial cells of the target animal using Cre-loxp gene knockout technology to construct The retinal neovascular disease model is as follows.

[0037] Mice carrying the loxp sequence (Ctnna1 loxp / loxp ) was mated with a mouse carrying Pdgfb-Cre to obtain a mouse carrying loxp sequence and Cre at the same time, denoted as Ctnna1 loxp / + - Pdgfb-Cre mice. Subsequently, Ctnna1 loxp / loxp mice with Ctnna1 loxp / + -Pdgfb-Cre mice were mated to obtain Ctnna1 loxp / loxp - Pdgfb-Cre mice. Please refer to the schematic diagram of knocking out the second exon of Ctnna1 in mouse vascular endothelial cells by using Cre-loxp technology in combination with the accompanying drawings. figure 1 In A, please refer to the breeding route map of Ctnna1 knockout mice figure 2 .

[0038] Ctnna1 loxp / loxp - Pdgfb-Cre mic...

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Abstract

The invention discloses a method and application for constructing a retinal neovascularization disease model, and relates to the field of biotechnology. Specifically, this method knocks out the Ctnna1 gene in the vascular endothelial cells of the target animal through gene editing technology, or silences the expression of the Ctnna1 gene in the vascular endothelial cells of the target animal through RNAi technology, so that the target animal shows typical retinal neogenesis The characteristics of vascular diseases, the disease model can be used as a disease model of new retinal blood vessels, and it provides convenience for the study of the pathogenesis and mechanism of retinal neovascular diseases, and also provides a new target for the treatment or prevention of the disease .

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and application for constructing a model of retinal neovascularization disease. Background technique [0002] Retinal neovascularization (RNV)-related eye diseases are a group of diseases characterized by pathological retinal neovascularization, mainly including proliferative diabetic retinopathy (PDR), retinopathy of prematurity (retinopathy) ofprematurity, ROP) and retinal vein occlusion (central retinal vei n occlusion, RVO) and so on. This type of disease has a high incidence rate and poor treatment prognosis, so it has a high blinding rate and seriously endangers human vision health. [0003] At present, there is no more effective treatment for RNV clinically except laser photocoagulation in the early stage of the disease and intraocular injection of anti-vascular endothelial growth factor (VEGF) drugs. Moreover, studies have shown that anti-VEGF therapy is not effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10C12N15/90C12N15/113C12Q1/02
CPCC07K14/47C12N5/069C12N15/113C12N15/907C12N2310/14C12N2503/02C12N2310/20G01N33/5064G01N2500/10
Inventor 杨正林朱献军李姝锦杨牧
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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