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Kit for prenatal screening of false positive by using peripheral blood fetal free DNA

A prenatal screening and peripheral blood technology, applied in the field of biomedicine, can solve the problems of long detection cycle, complicated experimental operation, and karyotype analysis technology alone, so as to ensure safety, ensure accuracy, and reduce pain. Effect

Pending Publication Date: 2020-06-16
CHANGZHOU MATERNAL & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps prevent premature birth by detecting if an individual has inherited specific genetic material from their own mother's body during gestation without requiring invasive procedures like biopsy. By comparing this data with other available tests for identifying abnormalities beforehand, it can help identify any potential problems that may affect future offspring health.

Problems solved by technology

This patents discuss various techniques used during embryonic development to diagnose certain conditions such as autism spectrum disorder by analyzing fetuses from their parents without carrying any risks associated therewith. These technical problem addressed include how to provide effective ways to identify specific types of chromoepitheliuas while minimizing potential complicating factors like abortion rates caused by other causes.

Method used

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  • Kit for prenatal screening of false positive by using peripheral blood fetal free DNA
  • Kit for prenatal screening of false positive by using peripheral blood fetal free DNA
  • Kit for prenatal screening of false positive by using peripheral blood fetal free DNA

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Embodiment Construction

[0022] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them.

[0023] A kit for prenatal screening of false positives using cell-free fetal DNA in peripheral blood, including peripheral blood DNA extraction reagents, DNA precipitation purification reagents, DNA end repair and linker reagents, DNA polymerase and DNA polymerase buffer, the kit The sequencing library was constructed by copy number variation analysis kit (Berry Genomics).

[0024] Further, the peripheral blood cell-free DNA includes maternal cell-free DNA extracted by any one of Chelex100 method, magnetic bead extraction method or organic extraction method.

[0025] Further, the DNA polymerase is KOD DNA polymerase, and the DNA polymerase buffer is KOD DNA polymerase buffer.

[0026] Further, the kit uses methylase-digested free DNA extr...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a kit for prenatal screening of false positive by utilizing peripheral blood fetal free DNA. The kit comprises a peripheral blood DNA extraction reagent, a DNA precipitation and purification reagent, a DNA terminal repair and linker reagent, DNA polymerase and a DNA polymerase buffer solution; according to the kit, a sequencing library is constructed through a copy number variation analysis kit, and when the calculation result of N is greater than 2, a pregnant woman does not need to carry out amniocentesis and otherhigh-risk detection methods, so that the safety of the pregnant woman and a fetus is guaranteed; when the calculation result of N is smaller than or equal to 2, the fetal sex chromosome needs to be further diagnosed, so that the prenatal screening accuracy of the peripheral blood fetal free DNA is ensured, the kit and the using method of the kit are convenient and simple, and the pain of pregnantwomen is relieved to a certain extent.

Description

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Claims

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Application Information

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Owner CHANGZHOU MATERNAL & CHILD HEALTH CARE HOSPITAL
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