Full-automatic integrated digital PCR (polymerase chain reaction) device

Abstract

The invention discloses a full-automatic integrated digital PCR (polymerase chain reaction) device which comprises a liquid drop generation component, a thermal cycle drainage channel, a thermal cyclecomponent, a fluorescence detection component and a controller. The thermal cycle component comprises a liquid drop slide, a mechanical arm, a high-temperature bath and a low-temperature bath; to-be-detected liquid drops generated by the liquid drop generation component flow through the thermal cycle drainage channel and flow into the liquid drop slide; the mechanical arm is used for circularly switching the liquid drop slide between the high-temperature bath and the low-temperature bath; the fluorescence detection component is used for performing fluorescence detection on the liquid drop slide after thermal cycle for preset times; the controller is used for controlling the liquid drop generation component, the thermal cycle component and the fluorescence detection component and quantitatively analyzing detection results. The to-be-detected liquid drops are more uniformly heated, and nucleic acid amplification inside the finally obtained to-be-detected liquid drops is more approximateto ideal conditions in experimental design, so that detection accuracy is higher.

Description

technical field [0001] The invention relates to the field of life sciences, in particular to a fully automatic integrated digital PCR device. Background technique [0002] DPCR (Digital PCR) is the abbreviation of digital PCR, which is a technology for the absolute quantification of nucleic acid molecules and the third generation of PCR technology. The number of DNA in the starting sample can be directly obtained by digital PCR technology. By dividing a sample into hundreds to tens of thousands of parts and assigning them to different reaction units, one or more units contain a target molecule (DNA template), and PCR amplification is performed on the target molecule in each reaction unit to amplify the target molecule. After the increase, the fluorescence signal of each reaction unit is statistically analyzed. By recording the number of DNA reaction units with fluorescence signals, it is possible to determine how many DNAs there are in the sample. By calculating the proport...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a fully automatic integrated digital PCR device to solve the problem of inaccurate temperature control of thermal cycle components in the prior art

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