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Lactobacillus paracasei with the ability to decompose oil and its application

A technology of lactobacillus and paracheese, applied in the field of lactic acid bacteria, can solve the problems of difficult to resist the taste and flavor of high-fat food

Active Publication Date: 2020-11-13
中科利君股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ways to reduce fat intake are mainly to change food cooking methods, reduce intake or use fat substitutes, etc. Although these methods can reduce fat intake to a certain extent, it is still difficult for people to resist the temptation of high-fat foods taste and flavor

Method used

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  • Lactobacillus paracasei with the ability to decompose oil and its application
  • Lactobacillus paracasei with the ability to decompose oil and its application
  • Lactobacillus paracasei with the ability to decompose oil and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 1. Strain screening

[0074] (1) Domestication of strains

[0075] Under aseptic conditions, use a pipette gun to draw appropriate amount of samples of five kinds of distiller's grains, which are marked as "S1, S2, S3, S4, S5" respectively, and inoculate these five kinds of distiller's grains samples into MRS and M17 medium, respectively. They were cultured in a constant temperature incubator at 37°C and 42°C, with a total of 20 parallel samples, and then the initially cultured strains were streaked and domesticated.

[0076] After 48 hours of acclimatization culture in MRS and M17 medium, the strain culture results in distiller's grains are as follows: figure 1 , as shown in Table 2, the colonies in only 16 petri dishes are more in line with the colony morphological characteristics of lactic acid bacteria in appearance, and the primary screening culture is further carried out.

[0077] Table 2 Strain acclimation results

[0078]

[0079] (2) Preliminary screening...

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Abstract

The invention discloses a strain of Lactobacillus paracasei F2, the preservation number of which is CGMCC No.18354. Lactobacillus paracasei F2 can degrade blend oil, peanut oil and sesame oil, and the degradation effect on peanut oil is the best, with a degradation rate of 27.12%. The strain has obvious lipase activity, the enzyme activity is as high as 5.88U / mL. At the same time, Lactobacillus paracasei F2 can degrade multiple fatty acids in peanut oil, especially fatty acids such as linoleic acid, oleic acid, stearic acid, palmitic acid, (Z)-hexadecenoic acid and eicosenoic acid. The total degradation rate of fatty acids in peanut oil can reach 4.65mg / mL. Lactobacillus paracasei F2 showed excellent acid and bile salt resistance in the tolerance test of artificial gastric juice, artificial intestinal juice and bile salt, and can tolerate human gastric juice and intestinal juice, and can be used as a probiotic with excellent performance to survive in the intestinal tract and play a role. The Lactobacillus paracasei F2 of the present invention can be used as a food additive or directly made into food, health products and the like.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria, and relates to a strain of Lactobacillus paracasei with the ability to decompose oil and its application. Background technique [0002] Dietary fat is an important source of energy for the human body and plays an important role in maintaining the energy balance of the human body. With the development of the economy, adequate food supply and other factors, dietary fat intake has increased significantly. Excessive dietary fat intake can promote the occurrence of chronic diseases such as obesity, coronary heart disease, diabetes and tumors. At present, the ways to reduce fat intake are mainly to change food cooking methods, reduce intake or use fat substitutes, etc. Although these methods can reduce fat intake to a certain extent, it is still difficult for people to resist the temptation of high-fat foods taste and flavor. [0003] After the fat is ingested, it mainly depends on the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L29/00A23C19/00A23L13/60A23L13/40A23L33/135A23L5/20C12R1/225
CPCC12N1/20A23L29/065A23C19/00A23L13/65A23L13/45A23L33/135A23L5/28C12R2001/225C12N1/205A23V2400/165
Inventor 陈大卫陶志强王丽关成冉顾瑞霞张臣臣黄玉军陈霞马文龙
Owner 中科利君股份有限公司
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