Interpretation method based on pathogen metagene next-generation sequencing
A technology of next-generation sequencing and metagenomics, applied in the field of interpretation based on second-generation sequencing of pathogenic metagenomics, can solve the problems of low reporting accuracy, no unified standard, and no unified standard for bioinformatics data interpretation, and achieve clinical operability Strong and low training investment
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[0031] The present invention will be further described below in conjunction with specific examples, but not as a limitation of the present invention.
[0032] The present invention is improved based on the summary and analysis of clinical data of large samples in the early stage, combined with relevant evidence of foreign coverage. The coverage of pathogens is the unity of the two parameters of sequence number and genome length, which can better interpret the abundance information of microorganisms.
[0033] This interpretation method includes:
[0034] A large number of / mainly detected sequences, meeting at least one of the following:
[0035] 1. Look at the coverage: the species level is more than 10 times that of any other species;
[0036] 2. Look at the abundance: more than 60%;
[0037] 3. Look at the sequence number: if the value of the total sequence number is normal, the SMRN or SDMRN exceeds 10,000;
[0038] Medium / minor detection sequence, at least one of the fo...
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