Biological control method of peanut root knot nematode

A technology for knot nematode and peanut root, which is applied in the field of biological control of peanut root knot nematode, can solve problems such as chemical damage, and achieve good insecticidal effect

Inactive Publication Date: 2019-09-06
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparations for the prevention and treatment of peanut root-knot nematode are mostly chemical formulations, but there are consistent chemical hazards. It can be seen that the development of a biological control method that can effectively prevent and control peanut root-knot nematode is very important for the green control of peanut root-knot nematode disease. of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The raw materials for the preparation of the composite microbial bacterial agent described in this embodiment include a composite microbial agent and a plant extract with a mass ratio of 1:3; wherein,

[0023] The composite bacterial agent is a mixed bacterial agent of Lactobacillus rhamnosus, Propionibacterium freunderi and Bacillus amyloliquefaciens with a mass ratio of 1:1:2;

[0024] The plant extract is prepared according to the following steps:

[0025] (1) Wash and peel the water chestnuts and burdock, and set aside; then mix the water chestnuts and burdock according to the mass ratio of 1:1, add water twice the amount of the total amount of raw materials, mix and make pulp, and set aside;

[0026] (2) Add cellulase accounting for 1 wt% of the total amount of the water chestnut and burdock raw materials to the above slurry, carry out enzymolysis treatment at room temperature for 3 hours, filter after inactivating the enzyme, collect the enzymolysis solution, spra...

Embodiment 2

[0029] The raw materials for the preparation of the composite microbial bacterial agent described in this embodiment include a composite microbial agent and a plant extract with a mass ratio of 1:8; wherein,

[0030] The composite bacterial agent is a mixed bacterial agent of Lactobacillus rhamnosus, Propionibacterium freunderi and Bacillus amyloliquefaciens with a mass ratio of 1:1:2;

[0031] The plant extract is prepared according to the following steps:

[0032] (1) Wash and peel the water chestnuts and burdock, and set aside; then mix the water chestnuts and burdock according to the mass ratio of 1:2, add 5 times the amount of water of the total amount of raw materials, mix and make pulp, and set aside;

[0033] (2) Add cellulase accounting for 2wt% of the total amount of water chestnut and burdock raw materials to the above slurry, carry out enzymolysis treatment at room temperature for 3 hours, filter after inactivating the enzyme, collect the enzymolysis solution, spra...

Embodiment 3

[0036] The raw materials for the preparation of the composite microbial bacterial agent described in this embodiment include a composite microbial agent and a plant extract with a mass ratio of 3:3; wherein,

[0037] The composite bacterial agent is a mixed bacterial agent of Lactobacillus rhamnosus, Propionibacterium freunderi and Bacillus amyloliquefaciens with a mass ratio of 1:1:2;

[0038] The plant extract is prepared according to the following steps:

[0039] (1) Take the water chestnuts and burdock, wash and peel them, and set aside; then mix the water chestnuts and burdocks according to the mass ratio of 1:1, add 3 times the amount of water of the total amount of raw materials, mix and make pulp, and set aside;

[0040] (2) Add cellulase accounting for 2wt% of the total amount of water chestnut and burdock raw materials to the above slurry, carry out enzymolysis treatment at room temperature for 3 hours, filter after inactivating the enzyme, collect the enzymolysis so...

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PUM

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Abstract

The invention belongs to the technical field of pest control, and particularly relates to a biological control method for peanut root knot nematode. The biological control method for the peanut root knot nematode comprises the steps that a composite microbial agent with the peanut root knot nematode control effect is applied to peanut planting soil, and by means of the good insecticidal effect ofthe composite microbial agent, the peanut root knot nematode is subjected to biological control. According to the composite microbial agent, the selected composite microbial agent and plant extracts serve as effectively ingredients, the synergistic effect of lactobacillus rhamnosus, propionibacterium freudenii and bacillus amyloliquefaciens and the effects of water chestnuts and burdock zymolyte are facilitated, all the ingredients play the insecticidal action through coordination, both an indoor test and a field test prove that the composite microbial agent has a good insecticidal effect on the peanut root knot nematode, and the growth situation of peanuts cannot be affected at all.

Description

technical field [0001] The invention belongs to the technical field of pest control, and in particular relates to a biological control method for peanut root-knot nematode disease. Background technique [0002] Peanut root-knot nematode disease, also known as peanut nematode disease, is commonly known as ground yellow disease, ground drop disease, dwarf yellow disease, yellow seedling disease, etc. The second-instar larvae of root-knot nematodes will invade the young root tips of peanuts and form irregular root knots. The nematodes will drill into the middle column of peanut roots, causing root fluid seepage and nutrient loss, which will easily cause secondary diseases. Root-knot nematodes colonize and feed and stimulate root cell proliferation, and continuously damage the root tips of new roots, causing secondary roots to form clusters (root clusters), making it difficult for them to absorb nutrients, and causing diseased plants to grow slowly or chlorosis. 1. The plant is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/40A01N63/00A01G13/00A01P5/00
CPCA01G13/00A01N63/00A01N65/00A01N65/12A01N65/40
Inventor 高宗军李如美李瑞娟戴争
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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