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Biological antifugal liquid preparation with microorganism pythium oligandrum and method of production

A technology of Pythium oligandrum and microorganisms, which is applied in the field of producing the product, can solve the problems of not being disclosed, and achieve the effect of simple application, long-term and significant stable effect

Pending Publication Date: 2019-07-30
BIOPREPARÁTY SPOL SRO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has not been disclosed a simple, well-tested and consistently defined method for Pythium oligandrum microorganisms or oospores with sufficient storability to produce regenerated forms that can be readily industrialized. Forms of preparation used

Method used

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  • Biological antifugal liquid preparation with microorganism pythium oligandrum and method of production
  • Biological antifugal liquid preparation with microorganism pythium oligandrum and method of production
  • Biological antifugal liquid preparation with microorganism pythium oligandrum and method of production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Liquid bioantifungal preparation for spraying crops with a high percentage by weight of biomass

[0066] preparation:

[0067] The biomass obtained by culturing the Pythium oligandrum M1 microorganism on a solid substrate was mixed with demineralized water and homogenized in a homogenizer vessel (industrial mixer). Homogenization was performed at 3000 to 5000 rpm for 3 minutes. Subsequently, permeate (sucrose) was added to the initial undiluted suspension in an amount corresponding to the resulting concentration of 60% by weight and corresponding to the desired number of oospores. The suspension with permeate (sucrose) was then homogenized in a mixer at 2000 revolutions per minute for 1 minute. The suspension was then stored in sterile stainless steel tanks at a temperature below 8°C.

[0068] It is more advantageous to prepare liquid bioantifungal preparations by combining the homogenization step in demineralized water and the subsequent addition of permeate (sucro...

Embodiment 2

[0075] Liquid bioantifungal preparation for spraying crops with a low weight percentage of biomass

[0076] preparation :

[0077] Pythium oligandrum biomass obtained by liquid culture was mixed with permeate (sucrose) in a vessel of a homogenizer (industrial mixer) and mixed at a concentration to achieve the desired number of oospores and permeate (sucrose) concentration. way of homogenization. Homogenization was performed at 3000 to 5000 rpm for 3 minutes. The suspension was then stored in sterile stainless steel tanks at a temperature below 8°C. The number of resting oospores was determined microscopically using a Sage counting plate counting chamber, and stability was measured using plasmolysis based on microscopic observation of the number of viable oospores according to a publication by Etxeberria et al. [13] in 2011. A preparation is marked as stable if the number of viable oospores does not fall below 90% of the initial value.

[0078] composition:

[0079] ...

Embodiment 3

[0083] Liquid biological antifungal preparations intended for use as seed coatings

[0084] preparation :

[0085] The biomass obtained by culturing the Pythium oligandrum M1 microorganism on a solid substrate was mixed with demineralized water and homogenized in a homogenizer vessel (industrial mixer). Homogenization was performed at 3000 to 5000 rpm for 3 minutes. Subsequently, permeate (sucrose) was added to the initial undiluted suspension in an amount corresponding to the resulting concentration of 60% by weight and corresponding to the desired number of oospores. The suspension with permeate (sucrose) was then homogenized in a mixer for 1 minute.

[0086] It is more beneficial to prepare preparations by combining the steps of homogenization in demineralized water and subsequent addition of permeate (sucrose) into one step, so that the biomass obtained by solid culture is in the container of an industrial homogenizer in the permeate solution (65% sucrose solution) fo...

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Abstract

The liquid biological antifungal product containing the Pythium oligandrum microorganism containing a stabilized suspension of the Pythium oligandrum microorganism contains 0.05 to 10.0 % weight culturing biomass of the Pythium oligandrum microorganism with content of cultivation medium, cell forms of this microorganism and substances produced by this microorganism and 90.0 to 99.95 % weight stabilizer, whereby the pre-determined number of dormant oospores in 1 ml of this liquid biological antifungal product is, following normal standardization, between 1*10<3> and 2*10<7>. The liquid biological antifungal product containing the Pythium oligandrum microorganism containing a stabilized suspension of the Pythium oligandrum microorganism contains 0.05 to 10.0 % weight culturing biomass of thePythium oligandrum microorganism with content of cultivation medium, cell forms of this microorganism and substances produced by this microorganism; and 79.77 to 99.95 % weight stabilizer, and the remainder, up to 100 % weight, a minimum of one modify ing / application substance from a group including filling, aroma and vitamin; whereby the pre-determined number of dormant oospores in 1 ml of thisliquid biological antifungal product is, following normal standardization, between 2.5*10<4> and 1.*<10<6>. The Pythium oligandrum microorganism is the Pythium oligandrum Dreschler ATTC 38472 strain,which was deposited in the Czech Collection of Microorganisms (CCM) at Masaryk University in Brno under the appellation of Pythium oligandrum M1. The stabilizer may be water, a salt solution, oil or aconcentrated solution of osmolytes. The method of production of this liquid biological antifungal product is claimed.

Description

technical field [0001] The present invention relates to liquid bioantifungal preparations containing Pythium oligandurm microorganisms. [0002] The invention also relates to methods of producing such articles. Background technique [0003] In recent decades, chemical antifungal preparations have raised increasing concerns in terms of their negative impact on the environment and substantially on users. Nevertheless, it can be said that the replacement of these products by effective biological products based on microorganisms capable of eliminating mold in a targeted and thoughtful manner has not gone as far as would be desired. Despite intensive research, only 14 microbial types are currently registered for this purpose in EU countries, as highlighted in a recent publication by Gerbore et al. [1] in 2014. [0004] The practical use of biological products using the antifungal action of the Pythium oligandrum microorganism has been known since the 1970s, for which we refer h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/22A01P3/00
CPCA01N63/00A01N63/30A01N65/03C12N1/105A61K35/68
Inventor M·沙涅克J·莫拉韦茨T·瓦内克A·斯蒂佩克
Owner BIOPREPARÁTY SPOL SRO
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